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当前位置: 首页 > 文章 > 苏丹红Ⅰ单克隆抗体的制备及其间接竞争ELISA检测 食品科学 2010,31 (18) 258-261
Position: Home > Articles > Monoclonal Antibodies against Sudan Ⅰ:Preparation of and Application in Indirect Competitive ELISA Detection FOOD SCIENCE 2010,31 (18) 258-261

苏丹红Ⅰ单克隆抗体的制备及其间接竞争ELISA检测

作  者：
裘雪梅;刘仁荣;徐玲;孟玮;涂顺;谢少霞
单  位：
江西科技师范学院生命科学学院
关键词：
苏丹红Ⅰ;单克隆抗体;ELISA检测
摘  要：
重氮法合成苏丹红Ⅰ的结构类似物,通过活性酯法将苏丹红Ⅰ的结构类似物偶联到载体蛋白上制备人工抗原,用所制备的人工抗原免疫BALB/c小鼠,采用细胞融合的方法制备苏丹红Ⅰ单克隆抗体,结果成功筛选到一株抗苏丹红Ⅰ单克隆抗体,经鉴定为IgG2a型,并建立间接竞争ELISA检测方法,线性范围为0.5～10ng/mL,检测下限为0.1ng/mL,加标回收率为52.3%～110%,变异系数为3.2%～8.9%。
译  名：
Monoclonal Antibodies against Sudan Ⅰ:Preparation of and Application in Indirect Competitive ELISA Detection
作  者：
QIU Xue-mei,LIU Ren-rong,XU Ling,MENG Wei,TU Shun,XIE Shao-xia(College of Life Science,Jiangxi Science and Technology Normal University,Nanchang 330013,China)
关键词：
Sudan Ⅰ;monoclonal antibody;ELISA detection
摘  要：
A SudanⅠanalog was synthesized by diazotization and conjugated to bovine serum albumin(BAS) or keyhole limpet hemocyanin(KLH) by active ester method and a BAS based detection antigen and a KLH based immune antigen were obtained.A competitive enzyme-linked immunosorbent assay(ELISA) was established to detect the immunogenity of the immune antigen.BALB/c mice were immunized with the immune antigen for antibody generation.For the preparation of anti-Sudan Ⅰ monoclonal antibodies,cell fusion was used.An indirect competitive ELISA assay was developed.A linear relationship was observed over the concentration range of 0.5 to 10 ng/mL and the detection limit was 0.1 ng/mL.The average recovery rates of Sudan Ⅰ in red hot chili pepper powder spiked at 6 different levels were between 52.3% and 110% with variation coefficients varying from 3.2% to 8.9%.

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