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当前位置: 首页 > 文章 > 杂交猪白细胞介素-18全基因的克隆与序列分析 畜牧与兽医 2007,39 (6) 1-3
Position: Home > Articles > Cloning and sequencing of interleukin-18 gene in an inbred pig Animal Husbandry & Veterinary Medicine 2007,39 (6) 1-3

杂交猪白细胞介素-18全基因的克隆与序列分析

作  者：
崔保安;郑兰兰;陈红英;方忠意;陈瑞亮;赵现敏;冯向杰
单  位：
河南农业大学牧医工程学院
关键词：
IL-18;克隆;序列分析;杂交猪
摘  要：
通过RT-PCR方法直接从猪脾脏淋巴细胞中扩增出猪白细胞介素-18(IL-18)全基因的cDNA,其大小为579bp,编码192个氨基酸。与GenBank上已发表猪IL-18序列(ABO10003)进行比较,核苷酸同源性为99.8%,在第550位处(以ATG为1计)由A→G,存在有意义突变。与GenBank上的ABO10003、AF176949、AY262109、NM1997序列进行比较分析,氨基酸同源性分别为99%,98.5%,99.8%和99%。从系统进化树可以看出,河南良杂猪IL-18基因与ABO10003、AY262109亲缘关系最近。河南良杂猪IL-18基因和人及其他动物IL-18基因核苷酸序列进行比较分析,结果显示猪与人、猫、牛、鸡、鸭、海豚、山羊、马、家鼠、老鼠、绵羊的IL-18基因核苷酸同源性分别为83.1%、88.3%、90.7%、26.8%、31.4%、26.3%、90.0%、91.5%、67.7%、65.1%和90.8%。
译  名：
Cloning and sequencing of interleukin-18 gene in an inbred pig
作  者：
CUI Bao-an1,2,ZHENG Lan-lan1,2,CHEN Hong-ying1,2,FANG Zhong-yi1,2,CHEN Rui-liang1,ZHAO Xian-min1,FENG Xiang-jie1(1.The College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China;2.Henan Animal Food Safety Key Labrotorary,Zhengzhou 450002,China)
关键词：
interleukin-18;gene cloning;sequencing;inbred pig
摘  要：
Interleukin18(IL-18)is a novel potent inducer of IFN-γ production.One pair of primers were designed according to porcine IL-18 gene sequence published in GenBank.IL-18 gene was amplified by RT-PCR from total RNA extracts of porcine splenocytes and then cloned and sequenced.The result indicated that the full length of porcine IL-18 gene was 579 bp,and encoded 192 amino acids.The nucleotide sequence of homology to the porcine IL-18 gene(Accession number:ABO10003)was 99.8%,and at the site of 550 bp the nucleotide changed from A to G.The deduced amino acid sequence of homologies to those of the porcine IL-18 genes(Accession number:ABO10003,AF176949,AY262109,NM1997)were 99%,98.5%,99.8% and 99%.Analysis of phylogenetic trees demonstrated that genetic relationship between the porcine IL-18 gene(ABO10003 and AY262109)were the closest.Comparison of the nucleotides sequence of porcine IL-18 gene with those from human,cat,cattle,chicken,duck,fugu,goat,horse,house mouse,rat and sheep showed that the nucleotides homologies were 83.1%,88.3%,90.7%,26.8%,31.4%,26.3%,90.0%,91.5%,67.7%,65.1% and 90.8%,individually.

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