当前位置: 首页 > 文章 > 基于线粒体标记的淮南麻黄鸡遗传结构及其遗传多样性分析 安徽农业大学学报 2018 (3) 400-403
Position: Home > Articles > The genetic diversity and population structure of Huainan yellow partridge chickens based on mitochondrial DNA marker Journal of Anhui Agricultural University 2018 (3) 400-403

基于线粒体标记的淮南麻黄鸡遗传结构及其遗传多样性分析

作  者:
黄胜海;贾晓旭;陆俊贤;唐修君;樊艳凤;葛庆联;高玉时
关键词:
淮南麻黄鸡;线粒体DNA;控制区;系统发育关系
摘  要:
通过探究淮南麻黄鸡的遗传多样性和遗传结构,为淮南麻黄鸡的种质资源保护和开发利用提供遗传背景资料。对淮南麻黄鸡线粒体基因组(mitochondrial genome,mt DNA)控制区全序列进行测序,并结合红色原鸡的序列进行生物信息学分析。淮南麻黄鸡mt DNA控制区全长1 231~1 232 bp,共发现26处单核苷酸变异位点,核苷酸多样性(Pi)为0.006 6,单倍型多样性(Hd)为0.917,平均核苷酸差异(K)为7.573。群体共包含14种单倍型,可以分为A、B、C和E 4个分支分别包括3、3、7和1种单倍型。淮南麻黄鸡在线粒体水平上有较高的多态性,并且来自多个不同的母系,有很好的开发潜力。
译  名:
The genetic diversity and population structure of Huainan yellow partridge chickens based on mitochondrial DNA marker
作  者:
HUANG Shenghai;JIA Xiaoxu;LU Junxian;TANG Xiujun;FAN Yanfeng;GE Qinglian;GAO Yushi;Jiangsu Institute of Poultry Science;
单  位:
HUANG Shenghai%JIA Xiaoxu%LU Junxian%TANG Xiujun%FAN Yanfeng%GE Qinglian%GAO Yushi%Jiangsu Institute of Poultry Science
关键词:
Huainan yellow partridge chickens;;mitochondrial DNA;;D-loop region;;phylogenetic relationship
摘  要:
This study was to investigate the genetic diversity and population structure of Huainan yellow partridge chickens. The complete D-loop region of mitochondrial DNA(mt DNA) of 32 chickens were sequenced and compared with published data of the red jungle fowl. The D-loop region of Huainan yellow partridge chickens was 1 231-1 232 bp in length. The average haplotype diversity, nucleotide diversity and nucleotide differences were 0.917, 0.006 6, and 7.573, respectively. A total of 14 haplotypes were identified, which belonged to four previously published clades, i.e. Clades A, B, C, and E, and contained 3, 3, 7, and 1 haplotypes, respectively. The results showed a high mitochondrial D-loop diversity and indicated multiple maternal origins of Huainan yellow partridge chickens. The results in this study will be a reference for conservation and utilization of Huainan yellow partridge chickens.

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