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当前位置: 首页 > 文章 > 鲈鱼肠道LactobacillussakeiLY1-6对荧光假单胞菌抑制作用研究 食品工业科技 2016,37 (23) 150-155
Position: Home > Articles > Inhibitory activity of Lactobacillus sakei LY1-6 from perch intestine against Pseudomonas fluorescens Science and Technology of Food Industry 2016,37 (23) 150-155

鲈鱼肠道LactobacillussakeiLY1-6对荧光假单胞菌抑制作用研究

作  者：
马欢欢;吕欣然;林洋;孙梦桐;白凤翎;励建荣
单  位：
北京林业大学生物科学与技术学院;渤海大学食品科学与工程学院辽宁省食品安全重点实验室生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心
关键词：
荧光假单胞菌;清酒乳杆菌LY1-6;海水鱼肠道;筛选;水产品保鲜
摘  要：
目的:从海鱼肠道中筛选对荧光假单胞菌具有较强抑制作用的乳酸菌。方法:采用牛津杯琼脂扩散法筛选菌株,通过生理生化反应和16S rRNA测序进行菌株生物学鉴定,利用蛋白酶、p H和温度等因素对抑菌活性进行分析,采用扫描电镜分析乳酸菌无细胞上清液(CFS)对荧光假单胞菌细胞结构的完整性影响。结果:从鲈鱼肠道中筛选出对荧光假单胞菌具有较强抑制活性的菌株LY1-6,经生理生化和16S rRNA测序鉴定为清酒乳杆菌(Lactobacillus sakei)。菌株LY1-6 CFS经胃蛋白酶处理后其抑菌活性完全丧失,经中性蛋白酶、木瓜蛋白酶、胰蛋白酶和碱性蛋白酶处理后其抑菌活性分别丧失了38.22%、38.00%、22.48%和18.46%。在p H2.5~5.0具有抑菌活性,并具有良好的热稳定性,初步结果表明LY1-6 CFS中抑菌活性物质可能为细菌素。扫描电镜结果表明经LY1-6 CFS处理使荧光假单胞菌细胞结构被破坏并溶解。结论:来自鲈鱼肠道的清酒乳杆菌LY1-6对荧光假单胞菌具有较强抑制作用,其抑菌活性物质为细菌素类,作用机制为破坏细胞的完整性,可作为控制水产品中荧光假单胞菌腐败生物保鲜的出发菌株。
译  名：
Inhibitory activity of Lactobacillus sakei LY1-6 from perch intestine against Pseudomonas fluorescens
作  者：
MA Huan-huan;LV Xin-ran;LIN Yang;SUN Meng-tong;BAI Feng-ling;LI Jian-rong;College of Food Science and Technology,Bohai University;Food Safety Key Lab of Liaoning Province;National & Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products;College of Biology Science and Technology,Beijing Forest University;
关键词：
Pseudomonas fluorescens;;Lactobacillus sakei LY1-6;;marine fish intestine;;screening;;seafood preservative
摘  要：
Objective: To isolate lactic acid bacteria( LAB) from marine fish intestine with strong antagonistic activity against Pseudomonas fluorescens. Methods: LAB strains with inhibitory activity against P. fluorescens were screened using the method of Oxford cup agar diffusion. The strains were identified by biochemical tests and16 S rRNA sequence analysis. The antibacterial substance was determined using protease sensitivity test,p H test and heat- treated test. The integrity of cell structure of P. fluorescens treated with cell-free supernatants( CFS) of produced by LY1-6 was observed by scanning electron microscope. Results: Strain LY1-6 with outstanding inhibitory activity against P. fluorescens was screened from ocean perch intestine,and was identified as Lactobacillus sakei.The inhibitory activity of LY1-6 CFS was completely lost by treated with pepsin,meanwhile,when treated with neutral protease,papaya protease,trypsin and alkaline protease,the inhibitory activity lost38.22%,38.00%,22.48% and 18.46% respectively. The inhibitory substance was effective within p H2.5 ~ 5.0 and stability after thermal treatment,which was preliminary determined as bacteriocins. The scanning electron microscope images revealed that the integrity of cell of P.fluorescens treated with LY1-6 CFS was damaged and dissolved.Conclusion: Lactobacillus sakei LY1-6 with strong inhibitory activity against P.fluorescens was screened from ocean perch intestine.Moreover,its antibacterial substance was determined as bacteriocins.The mechanism of action was destruction the integrity of cell.Lb.sakei LY1-6 biological preservative can be used as starting strain for the control of P.fluorescens in aquatic products spoilage.

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