中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 野生毛葡萄“花溪-4”试管苗对PEG胁迫的形态及生理响应 西北农林科技大学学报(自然科学版) 2012 (6) 181-188
Position: Home > Articles > Response of wild Vitis quinquangularis"Huaxi-4" tube seedlings to PEG stress in morphology and physiology Journal of Northwest A & F University(Natural Science Edition) 2012 (6) 181-188

野生毛葡萄“花溪-4”试管苗对PEG胁迫的形态及生理响应

作  者：
仲伟敏;潘学军;刘伟;周金忠
单  位：
贵州省果树工程技术研究中心;贵州大学农学院
关键词：
野生毛葡萄;PEG胁迫;试管苗;旱害指数;保护酶
摘  要：
【目的】研究毛葡萄"花溪-4"试管苗在聚乙二醇(PEG)胁迫下的形态和生理响应,为毛葡萄苗期抗旱性鉴定提供依据。【方法】以毛葡萄单株"花溪-4"试管苗为试材,通过在培养基中加入不同质量浓度(0,30,60,90g/L)PEG-6000模拟干旱胁迫,研究"花溪-4"外观形态、保护酶系统、过氧化氢(H2O2)和丙二醛(MDA)含量的变化。【结果】不同质量浓度PEG胁迫对"花溪-4"试管苗生长的抑制程度不同,其质量浓度越大、胁迫时间越长,旱害越严重,对"花溪-4"试管苗生长的抑制效果越明显。随着PEG质量浓度的升高和胁迫时间的延长,"花溪-4"叶片中的MDA和H2O2含量均逐渐上升,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性则呈先上升后下降的变化趋势,且各生理指标峰值的出现时间与PEG质量浓度的高低相关,其质量浓度越高,峰值出现越早。MDA和H2O2含量与旱害指数(DI)呈极显著正相关,POD与DI呈极显著负相关,而SOD、CAT与DI相关性不显著。【结论】PEG胁迫会诱导"花溪-4"试管苗叶片内的H2O2含量上升,膜脂过氧化加重,而POD在试管苗活性氧清除中起主要作用;随PEG胁迫程度的加剧和胁迫时间的延长,"花溪-4"试管苗生长所受到的抑制作用增强。
译  名：
Response of wild Vitis quinquangularis"Huaxi-4" tube seedlings to PEG stress in morphology and physiology
作  者：
ZHONG Wei-min1,2,PAN Xue-jun1,2,LIU Wei1,2,ZHOU Jin-zhong1,2(1 Guizhou Engineering Research Center for Fruit Crops,Guiyang,Guizhou 550025,China; 2 Agricultural College of Guizhou University,Guiyang,Guizhou 550025,China)
关键词：
wild Vitis quinquangularis;PEG stress;tube seedling;drought index;protective enzyme
摘  要：
【Objective】 For the purpose of providing basis for drought resistance of wild Vitis quinquangularis seedlings,the response of the tube seedlings of wild Vitis quinquangularis in morphology and physiology was studied under different concentrations of polyethylene glycol(PEG-6000).【Method】 Taking the tube seedlings of Vitis quinquangularis "Huaxi-4" as materials,the changes in morphology,the activities of protective enzymes and the contents of H2O2 and MDA were studied under drought stress by PEG.【Result】 Drought stress,induced by PEG,had different inhibition actions on the growth of grape plantlets in vitro.In the meanwhile,drought hazard and growth inhibition were raised remarkably by adding concentrations and stress time.Along with different concentrations and long-term PEG stress,the contents of MDA and H2O2 were gradually increased,while the protective enzymes activities of SOD,POD and CAT,in the rooting tube seedlings,ascended first and then descended;the occurrence of the peak of protect enzymes was earlier with increased concentrations of PEG.There was a significant positive correlation between the contents of MDA and H2O2 and the index of drought hazard,and a significant negative correlation between the protective enzymes activities of POD and the index of drought hazard,but an insignificant correlation between the activities of SOD and CAT and the index of drought hazard.【Conclusion】 Along with different concentrations and long-term PEG stress,drought stress enhanced the inhibition actions of the growth on the tube seedlings of "Huaxi-4",and could induce increase in the contents of H2O2 and promote the peroxide of membrane lipid as well as the contents of MDA,while the contents of H2O2 served a primary function for ROS-eliminating system.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46