当前位置: 首页 > 文章 > IPT基因遗传转化谷秆两用稻的研究 福建农林大学学报(自然科学版) 2003,32 (2) 205-208
Position: Home > Articles > A study on genetic transformation of grain-straw-dual-use-rice with IPT gene Journal of Fujian Agriculture and Forestry University(Natural Science Edition) 2003,32 (2) 205-208

IPT基因遗传转化谷秆两用稻的研究

作  者:
许明;刘峰;郑金贵
单  位:
福建农林大学生命科学学院
关键词:
谷秆两用稻;农杆菌;IPT;遗传转化
摘  要:
采用农杆菌介导的方法,将嵌合基因PSAG12-IPT导入谷秆两用稻201幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得46个抗性愈伤克隆,126株转基因植株.对这些植株进行PCR检测和GUS染色分析,确定其中80株为阳性植株.
译  名:
A study on genetic transformation of grain-straw-dual-use-rice with IPT gene
作  者:
XU Ming, LIU Feng, ZHENG Jingui (College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)
关键词:
grain-straw-dual-use-rice; Agrobacterium; IPT gene;genetic transfomation
摘  要:
Chimeric gene PSAG12IPT was transformed into embryonic calli induced from grainstrawdualuserice 201 juvenile embryo by Agrobacteriummediated method. After resistance screening of hygromycin and differentiation and growth of plantlets, 46 clones of resistance calli and 126 transgenic plants were obtained. Results of PCR detection and GUS staining analysis showed that 80 of those plants were positive.

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