中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 白苏叶黄酮的微波辅助提取及其纯化的研究 食品工业科技 2016,37 (6) 280-286
Position: Home > Articles > Study on microwave-assisted extraction and purification of flavonoids from Perilla frutescens leaves Science and Technology of Food Industry 2016,37 (6) 280-286

白苏叶黄酮的微波辅助提取及其纯化的研究

作  者：
杜艳;李荣;姜子涛
单  位：
天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室
关键词：
黄酮;白苏叶;响应面设计;大孔树脂;制备色谱
摘  要：
目的:研究白苏叶黄酮的微波提取与纯化工艺。方法:通过响应面设计和单因素实验法确定白苏叶黄酮的最佳提取条件;采用AB-8大孔树脂和制备色谱两种方式纯化白苏叶黄酮。结果:最佳提取条件为:乙醇体积分数63%、液料比41∶1(m L/g)、微波温度72℃、微波起始功率500 W和提取时间7 min,此条件下的黄酮提取得率为7.81%。最佳大孔树脂纯化条件为:吸附流速2 BV/h、样品液的p H为2、洗脱液乙醇体积分数为70%、洗脱流速3 BV/h,纯化后的总黄酮纯度由21.24%提高到53.10%。制备色谱可将白苏叶黄酮成分粗分为2个组分。结论:响应面法优化白苏叶黄酮提取工艺,方便有效,大孔树脂和制备色谱用于纯化白苏叶黄酮具有可行性。
译  名：
Study on microwave-assisted extraction and purification of flavonoids from Perilla frutescens leaves
作  者：
DU Yan;LI Rong;JIANG Zi-tao;Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce;
关键词：
flavonoids;;Perilla frutescens(L.) Britt.leaves;;response surface design;;macroporous resin;;preparative chromatography
摘  要：
Objective : To study the extraction process of Perilla frutescens( L.) Britt. leaves flavonoids and purification process. Methods:The extraction conditions of flavonoids of Perilla frutescens(L.) Britt. leaves were optimized by response surface design and single factor experiment. The flavonoids from Perilla frutescens(L.)Britt. leaves were purified by AB-8 macroporous resin and preparative chromatography. Results:The optimal extraction conditions were:ethanol concentration,ratio of liquid to material,extraction temperature,initial microwave power, and extraction time were 63 %, 41 ∶ 1(m L / g), 72 ℃, 500 W and 7 min, respectively. Under these conditions,the yield of flavonoids was 7.81%. The optimal purification conditions of macroporous resin were:the sample solution passed through the resin column at a flow rate of 2 BV/h and p H2. Subsequently,the column was eluted by 70% alcohol at a flow rate of 3 BV/h. The purity of flavonoids increased from 21.24% to 53.10%.The flavonoids were divided into two groups by preparative chromatography. Conclusion:The optimum extraction technology of flavonoids from Perilla frutescens( L.) Britt. leaves by response surface was convenient and effective. The results indicated that the feasibility of macroporous resin and preparative chromatography for purification of flavonoids.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46