中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 奥尼罗非鱼杂交子代及亲本mtDNA D-loop基因序列的多态性 广东海洋大学学报 2009,29 (6) 12-15
Position: Home > Articles > Polymorphism of the Mitochondrial DNA D-loop Gene Sequences of Oreochromis niloticus,O. aureus and Their Hybrids Journal of Guangdong Ocean University 2009,29 (6) 12-15

奥尼罗非鱼杂交子代及亲本mtDNA D-loop基因序列的多态性

作  者：
李培;刘丽;谭围;刘楚吾
单  位：
广东海洋大学水产学院
关键词：
奥利亚罗非鱼;尼罗罗非鱼;杂交子代;线粒体DNA;D-loop;多态性
摘  要：
利用PCR技术扩增奥利亚罗非鱼(Oreochromis aureus)、尼罗罗非鱼(O.niloticus)及其杂交子代(O.aureus♂×O.niloticus♀)3个群体mtDNA控制区全序列,分析其变异和遗传结构。结果显示:3个群体的mtDNA控制区序列长度多态性并不十分明显,全长921～933bp;3个群体间存在丰富的DNA序列多态性,共检测到138个变异位点(14.9%),44个个体具有44种单倍型(haplotype);3群体间的序列差异平均为4%,Tamura-Nei遗传距离平均为5.1%;采用NJ法和ME法构建的分子系统树中,奥利亚罗非鱼群体内所有的单倍型聚成一支,尼罗罗非鱼与其杂交子代的单倍型混杂在一起,聚成一支,表明杂交后代mtDNAD-loop表现为母系遗传。
译  名：
Polymorphism of the Mitochondrial DNA D-loop Gene Sequences of Oreochromis niloticus,O. aureus and Their Hybrids
作  者：
LI Pei, LIU Li, TAN Wei, LIU Chu-wu (Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China)
关键词：
Oreochromis aureus; O. niloticus; hybrids; mtDNA D-loop; polymorphism
摘  要：
The complete sequences of mitochondrial DNA control region in tilapia, Oreochromis aureus (OL), O. niloticus (NL)and the hybrid of O. aureus ♂×O. niloticus♀(AN) were determined to analyze their sequence variation and genetic structure. The sequence length of the control region in individuals of tilapia was between 921bp and 933bp, which revealed that the length polymorphism of control region sequence in tilapia was not distinct, but high nucleotide content polymorphism was examined in the three populations. 138 loci (14.9%) were variable in mtDNA D-loop gene segments,and there were 44 haplotypes in 44 individuals. The average sequence divergence of control region in three tilapia populations was 4%, and the Tamura-Nei distances was 5.1%. In molecular phylogenetic tree constructed by NJ method and ME method, all the haplotypes of Oreochromis aureus samples clustered into one branch, while the haplotypes of Oreochromis niloticus and the hybrids samples intermixed together and clustered into another branch, which showed that mtDNA D-loop of the hybrids presented maternal inheritance.

相似文章

导出引用
计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46