当前位置: 首页 > 文章 > 绒山羊胚胎期和兴盛期皮肤毛囊差异表达基因研究 湖北农业科学 2008,47 (2) 146-148
Position: Home > Articles > Study on Differently Expressed Genes in Skin Follicle of Cashmere Goat at the Embryonic Stage and Anagen Stage Hubei Agricultural Sciences 2008,47 (2) 146-148

绒山羊胚胎期和兴盛期皮肤毛囊差异表达基因研究

作  者:
李玉荣;尹俊;张燕军;刘羿羿;李金泉
单  位:
内蒙古农业大学动物科学与医学学院;内蒙古农业大学生物工程学院
关键词:
抑制性消减杂交;绒山羊;胚胎期;兴盛期;消减cDNA文库
摘  要:
应用抑制性消减杂交技术成功构建了内蒙古绒山羊胚胎期和兴盛期皮肤毛囊的消减cDNA文库。从两个文库中分别随机挑取50个克隆测序分析,测序结果经Genbank同源性比对,表明在胚胎期消减cDNA文库中得到15个特异表达基因片段,包含1个未知新序列;在兴盛期消减cDNA文库中得到19个特异表达基因片段,包含2个未知新序列。
译  名:
Study on Differently Expressed Genes in Skin Follicle of Cashmere Goat at the Embryonic Stage and Anagen Stage
作  者:
LI Yu-rong1a,2,YIN Jun1b,ZHANG Yan-jun1a,LIU Yi-yi1a,LI Jin-quan1a (1.Inner Mongolia Agricultural University a.College of Animal Science and Medical Science;b.Institute of Bioengineering,Huhehaote 010018,China;2.Institute of Animal Sciences,Inner Mongolia Academy of Angriculture and Animal Husbandry Sciences,Huhehaote 010018,China)
关键词:
suppression subtractive hybridization;cashmere goat;embryonic stage;anagen;subtractive cDNA library
摘  要:
The skin follicle subtractive cDNA library of the Inner Mongolian cashmere goat was constructed by suppression subtractive hybridization.50 clones which were derived from the embryonic stage and anagen cDNA library respectively were sequenced and blasted with known gene in Genbank.15 cDNA fragments were obtained from the embryonic stage cDNA library,14 fragments corresponded to known gene and one was unknown sequence.19 cDNA fragments were obtained from anagen cDNA library,17 cDNA fragments corresponded to known gene and two was unknown sequence.

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