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当前位置: 首页 > 文章 > 响应面法优化秸秆发酵产β-葡萄糖苷酶的研究 河北农业科学 2015 (1) 80-83+94
Position: Home > Articles > Optimization of Straw Fermentation Conditions for Production of β-Glucosidase with Response Surface Methodology Journal of Hebei Agricultural Sciences 2015 (1) 80-83+94

响应面法优化秸秆发酵产β-葡萄糖苷酶的研究

作  者：
王敏;王颉
单  位：
河北农业大学食品科技学院;衡水学院生命科学系
关键词：
β-葡萄糖苷酶;Plackett-Burman;Center Composite Design;康氏木霉;秸秆
摘  要：
为了高效利用废弃的农作物秸秆,优化康氏木霉固态发酵秸秆生产β-葡萄糖苷酶的发酵参数,首先利用Plackett-Burman试验,对影响生产β-葡萄糖苷酶的参数麸皮添加量、(NH4)2SO4添加量、培养温度、培养时间、料水比、装料比、浸提液p H值进行了主要影响因子的筛选;然后再利用Center Composite Design响应面分析对产酶参数进行了优化。结果表明:培养温度和培养时间是影响固态发酵生产β-葡萄糖苷酶的主要因素;经CCD优化后,得到产酶最适条件为培养时间6.6 d、培养温度27℃,该条件下β-葡萄糖苷酶活性为77.68 IU/m L。验证试验证实,预测回归方程的预测值与试验值之间具有较高的拟合度。本研究结果可为农业秸秆资源的再利用和纤维素酶的工业化生产提供科学依据。
译  名：
Optimization of Straw Fermentation Conditions for Production of β-Glucosidase with Response Surface Methodology
作  者：
WANG Min;WANG Jie;Department of Life Science,Hengshui University;College of Food Science and Technology,Agricultural University of He Bei;
关键词：
β-glucosidase;;Plackett-Burman;;Center Composite Design;;Trichoderma koningii;;Straw
摘  要：
For more efficient utilization of wasted crop straw,fermentation parameters of Trichoderma koningii which can produce β-glucosidase were optimized. First,screen the factors that affect the production of β-Glucosidase with Plackett-Burman test,which including addition level of bran and( NH4)2SO4,culture temperature,culture time,material-water ratio, loading ratio, p H of leaching liquor, then production parameters on enzyme were optimized with the Center Composite Design of response surface analysis. The results showed that the main factors were culture temperature and time for β-glucosidase production on solid fermentation with straw. After optimizing by CCD,get the optimal conditions for Trichoderma koningii were the culture time 6. 6 d,culture temperature 27 ℃.At the conditions, β-glucosidase enzyme activity was 77. 68 IU / m L. Verification test showed that prediction regression equation had higher degree of fitting between prediction values and experimental values. The research result can provide scientific basis for the recycle of agricultural straw resources and the industrialization of the cellulose enzyme.

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