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当前位置: 首页 > 文章 > 椰子织蛾转录组分析 西南林业大学学报(自然科学) 2018 (5) 38-45
Position: Home > Articles > Transcriptome Analysis of Opisina arenosella Journal of Southwest Forestry University(Natural Sciences) 2018 (5) 38-45

椰子织蛾转录组分析

作  者：
徐志文;任雪敏;王俊;张枝全;刘乃勇;阎伟;朱家颖
关键词：
椰子织蛾;转录组;基因;功能注释
摘  要：
为获取入侵性检疫害虫椰子织蛾的基因信息,利用高通量测序技术对该害虫进行了转录组测序。结果表明:经拼接共获得了37 416条unigenes,平均长度为803 bp,N50为1 415 bp;通过BlastX比对,有19 154条unigenes (51.19%)成功在Nr中被注释。在Nr中成功注释的unigene,与黑脉金斑蝶同源性最高,达到68.45%;在GO注释中,共有14 131 (37.76%)条unigenes被成功注释到细胞组分、分子功能和生物过程3大类的54个分支;在COG数据库中,成功注释的8 606条unigenes被注释到26个分类;在KEGG中,共有6 171条(16.49%) unigenes被注释到5大类通路的260条途径上。基于构建的转录组数据库,鉴定得到3 248个微卫星。
译  名：
Transcriptome Analysis of Opisina arenosella
作  者：
Xu Zhiwen;Ren Xuemin;Wang Jun;Zhang Zhiquan;Liu Naiyong;Yan Wei;Zhu Jiaying;Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Southwest Forestry University;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences;
单  位：
Xu Zhiwen%Ren Xuemin%Wang Jun%Zhang Zhiquan%Liu Naiyong%Yan Wei%Zhu Jiaying%Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Southwest Forestry University%Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences
关键词：
Opisina arenosella;;transcriptome;;gene;;functional annotation
摘  要：
In order to obtain genetic data for Opisina arenosella,this study sequenced its transcriptome using high-throughput sequencing technology.In total,37 416 unigenes were obtained.Their mean length was 803 bp with N50 of 1 415 bp.By BlastX search,a total of 19 154 unigenes were matched to the sequences in NCBI non-redundant protein sequences( Nr) database.They had the highest similarity( 68.45%) to those of Danaus plexippus.In Gene Ontology( GO) database,14 131 unigenes( 37.76%) were assigned to 54 branches of 3 categories including cellular component,biological processes and molecular function.In Clusters of Orthologous Groups of proteins( COG) database,8 606 unigenes were assigned to 26 categories.Additionally,6 171 unigenes( 16.49%) were mapped to Kyoto Encyclopedia of Genes and Genomes( KEGG) database,of which were divided into 260 known pathways of 5 metabolic pathways categories.In total,3 248 microsatellites were identified from the transcriptome database.

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