中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 磺胺甲噁唑与吉富罗非鱼血浆蛋白结合率的测定方法研究淡水渔业 2018 (6) 53-60

Position: Home > Articles > Study on the determination method of plasma protein binding rate of sulfamethoxazole in Oreochromis niloticus Freshwater Fisheries 2018 (6) 53-60

磺胺甲噁唑与吉富罗非鱼血浆蛋白结合率的测定方法研究

作者：

王赛赛;宋怿;程波

单位：

上海海洋大学水产与生命学院;中国水产科学研究院农业部水产品质量安全控制重点实验室

关键词：

磺胺甲噁唑;血浆蛋白结合率;体外平衡透析法;体内超滤法;吉富罗非鱼(Oreochromis niloticus)

摘要：

为建立磺胺甲噁唑(Sulfamethoxazole,SMZ)与吉富罗非鱼(Oreochromis niloticus)血浆蛋白结合率的测定方法,实验研究了体外平衡透析法测定SMZ血浆蛋白结合率的最佳测定条件,从而推断出体内超滤法的最佳测定条件,最后进行了两种测定方法的对比研究。结果显示:SMZ血浆蛋白结合率不受温度影响,但具有p H依赖性和药物浓度依赖性。在4℃、p H 7. 4条件下,体内外血浆蛋白结合率测定结果如下:0. 5～10. 0μg SMZ/m L透析液范围内,体外SMZ血浆蛋白结合率为15. 48%～25. 12%,10. 0～60. 0μg SMZ/m L透析液内,体外血浆蛋白结合率维持在14. 65%～15. 48%内;在血浆含药浓度为1～5μg/m L内,体内SMZ血浆蛋白结合率为15. 34%～20. 1%;在10～20μg/m L内,体内结合率趋于稳定,维持在12. 7%～14. 9%内,与体外测定结果的变化趋势保持一致。通过两种测定方法的对比研究,确定体内超滤法为SMZ血浆蛋白结合率测定的最佳选择。

译名：

Study on the determination method of plasma protein binding rate of sulfamethoxazole in Oreochromis niloticus

作者：

WANG Sai-sai;SONG Yi;CHENG Bo;College of Fisheries and Life Science,Shanghai Ocean University;Chinese Academy of Fishery Sciences,Key Labrotary of Control of Quality and Safety for Aquatic Products,Minisrty of Agriculture;

关键词：

sulfamethoxazole;;plasma protein binding rate;;in vitro equilibrium dialysis;;in vivo ultrafiltration;;Oreochromis niloticus

摘要：

The optimal conditions for determination of SMZ plasma protein binding rate by in vitro equilibrium dialysis were studied to establish a method for the determination of plasma protein binding of sulfamethoxazole in Oreochromis niloticus. A comparative study of the two methods was performed. The results showed that plasma protein binding of SMZ was temperature independent,p H dependent and concentration dependent. The results of the in vitro and in vivo plasma protein binding assays at 4 ℃ and p H 7. 4 were tested. In vitro SMZ plasma protein binding rates were ranged from 15. 48% to 25. 12% by dialysate of 0. 5 ～ 10. 0 μg SMZ/m L. In vitro SMZ plasma protein binding rates were ranged from14. 65% to 15. 48% by dialysate of 10. 0 ～ 60. 0 μg SMZ/m L. The plasma protein binding rate of SMZ in vivo was ranged from 15. 34% to 20. 1%and from 12. 7% to 14. 9% by plasma concentration of 1 ～ 5 μg SMZ/m L and 10 to 20 μg SMZ/m L, respectively. Consistent with the trend of changes in vitro measurements. As a conclusion,this experiment determined that the best choice for the determination of SMZ plasma protein binding rate was in vivo ultrafiltration by the comparative study of the two assay methods.

相似文章

利用表面等离子共振技术快速检测牛奶中的磺胺甲噁唑 [周宏敏, 欧惠超, 姜浩, 江海峰, 王晓萍, 罗昭锋] 食品科学 2010,31 (6) 168-171
磺胺甲噁唑单克隆抗体的制备及初步鉴定 [吴红军, 吴国娟, 张春叶, 孙玉成, 曹以福] 兽药与饲料添加剂 2007,12 (1) 1-3
高效液相色谱法测定欧洲鳗鲡肌肉中磺胺嘧啶和磺胺甲噁唑残留 [林丽聪] 福建农业学报 2011,26 (5) 17-20
磺胺甲噁唑抗原合成及其抗体的制备 [沈红, 吴国娟, 李焕荣] 动物医学进展 2006,27 (1) 76-79
磺胺甲噁唑与吉富罗非鱼血浆蛋白结合率的测定方法研究 [王赛赛, 宋怿, 程波] 淡水渔业 2018,48 (6) 53-60
水产品中磺胺甲噁唑与甲氧苄啶含量的高效液相色谱测定法 [刘长征, 艾晓辉, 伍刚, 袁科平] 淡水渔业 2004,34 (2) 23-24
磺胺甲噁唑残留检测阻断ELISA试剂盒的研制 [刘宣兵, 张改平, 王斌, 滕曼, 邓瑞广, 侯玉泽, 邢广旭, 杨继飞] 华北农学报 2009,24 (2) 214-217
流动注射和电化学联用测定磺胺甲恶唑含量 [孙彬彬, 宋蓉, 丁开创] 绿色科技 2010 (7) 179-181
磺胺甲噁唑的控温相变免疫分析 [沈红, 吴国娟, 苏萍, 郭振泉] 中国兽医科学 2006,36 (4) 336-338
磺胺甲噁唑残留快速检测胶体金试纸条的研制 [裴兆柱, 郭玮, 周旭平, 吴国娟] 中国兽医杂志 2008,044 (9) 90-92

导出引用

计量

文章访问数: 16

HTML全文浏览量: 0

PDF下载量: 0

磺胺甲噁唑与吉富罗非鱼血浆蛋白结合率的测定方法研究

相似文章

所属期刊

淡水渔业

推荐期刊

动物医学进展

淡水渔业