当前位置: 首页 > 文章 > 犬血浆中托芬那酸高效液相色谱检测法的建立 中国兽医杂志 2016,50 (6) 52-55
Position: Home > Articles > Determination of Tolfenamic Acid in Dog by High Performance Liquid Chromatography Chinese Journal of Veterinary Medicine 2016,50 (6) 52-55

犬血浆中托芬那酸高效液相色谱检测法的建立

作  者:
李龙飞;李宇琛;吴天兴;夏良友;曹杰;王双;卜仕金
单  位:
扬州大学兽医学院
关键词:
高效液相色谱;托芬那酸;犬血浆
摘  要:
为了建立高效液相色谱(HPLC)法测定地犬血浆中托芬那酸的含量,采用 Agilent ZOR-BAX Eclipse Plus C18(250 mm ×4.6 mm,5μm)色谱柱,流动相使用甲醇∶乙腈∶0.05 M醋酸钠(pH =4.6)为36.5∶36.5∶27,流速为0.7 mL/min,柱温为35℃,检测波长为290 nm。结果:托芬那酸的血浆药物浓度在0.05~10.0μg/mL 范围内,线性关系良好(r =0.9993);最低检出限为0.025μg/mL;定量限为0.05μg/mL,平均回收率为85%,批内和批间系数分别小于11%和6%。本方法简便准确、快速可靠,适用于犬血浆中托芬那酸的药动学和相对生物利用度研究。
译  名:
Determination of Tolfenamic Acid in Dog by High Performance Liquid Chromatography
关键词:
HPLC%tolfenamic acid%dog plasma
摘  要:
To establish an HPLC method for quantification of tolfenamic acid in dog.The separation was performed on an Agilent ZORBAX Eclipse Plus C18 (250 mm ×4.6 mm,5 μm).Mobile phase:methanol ∶acetonitrile∶0.05 Msodium acetate (pH =4.6)was 4.6∶36.5∶27.The column temperature was 35 ℃ and the detection wavelength was set at 290 nm.The velocity was 0.7 mL/min.Results:A good linear correlation was observed within the range of 0.05 ~10.0 μg/mL for tolfenamic acid (r =0.9993),the minimum detection limit was 0.025 μg/mL,limit of quantitation was 0.05 μg/mL;The average recovery was 85%.Within -run and between -run analysis less than 11% and 6% respectively.The method is simple,accurate and rapid.It could be suitable for dogs tolfenamic acid in plasma pharmacokinetic experiments.

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