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当前位置: 首页 > 文章 > 苯酚对红鳍东方幼鱼的急性毒性及对肝脏抗氧化酶的影响 吉林农业大学学报 2012,34 (6) 95-100+117
Position: Home > Articles > Effects of Phenol on Hepatic Antioxidant Enzymes and Acute Toxicity in Juvenile Takifugu rubripes Journal of Jilin Agricultural University 2012,34 (6) 95-100+117

苯酚对红鳍东方幼鱼的急性毒性及对肝脏抗氧化酶的影响

作  者：
石洪玥;姜志强;高小强;宫雪;吴洪;李艳秋
单  位：
大连海洋大学
关键词：
苯酚;红鳍东方;急性试验;亚急性试验;抗氧化酶
摘  要：
采用静水生物测试法研究了苯酚对红鳍东方(Takifugu rubripes)幼鱼的急性毒性并进行安全评价。试验鱼体质量为(7.50±1.09)g,全长(7.92±1.38)cm。根据预试验结果,急性试验设定苯酚质量浓度梯度为0,3.2,6.5,10.0,13.2,16.5,20.0 mg/L,每组试验浓度分别暴露24,48,96 h。亚急性试验设定质量浓度梯度为0,0.18,0.32,0.56,1.00,1.35 mg/L,每组试验浓度分别暴露4,8,12,16 d,以肝脏谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)和谷胱甘肽-S转移酶(GST)活性为指标,研究了苯酚污染对红鳍东方幼鱼的毒理作用。结果表明:在急性试验中,苯酚质量浓度的升高会对红鳍东方幼鱼产生较大毒性,暴露24,48,96 h的LC50分别为17.56,15.41,12.09 mg/L,安全质量浓度为1.21 mg/L。亚急性试验,不同苯酚浓度处理组的红鳍东方幼鱼在暴露16 d时,肝组织中SOD和GST的活性均表现出被显著抑制(P<0.05)。随着暴露时间的延长,GSH-PX的活性均表现出先升高再降低的趋势,其活力最高值由高浓度组逐渐移向低浓度组;与对照组(0 mg/L)相比,各组总SOD活性随着暴露时间的延长,呈现先降低后升高再下降的趋势,除0.18 mg/L浓度组外,其他浓度组在暴露16 d时,与对照组相比均差异显著(P<0.05);各浓度组的GST活性随暴露时间的延长基本呈现逐渐降低的趋势。
译  名：
Effects of Phenol on Hepatic Antioxidant Enzymes and Acute Toxicity in Juvenile Takifugu rubripes
作  者：
SHI Hong-yue,JIANG Zhi-qiang,GAO Xiao-qiang,GONG Xue,WU Hong,LI Yan-qiuKey Laboratory of Mariculture & Stock Enhancement in North China′s Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,China
关键词：
phenol;Takifugu rubripes;acute experiment;sub acute experiment;antioxidant enzyme
摘  要：
The acute toxicity of phenol and the changes of antioxidant enzyme of Takifugu rubripes juvenile with weight of(7.50±1.09) g and total length of(7.92±1.38) cm were studied by the static test method.The acute experimental concentrations of phenol were set up at 0,3.2,6.5,10.0,13.2,16.5 and 20.0 mg/L,and the exposure time were set up at 24 h,48 h and 96 h.The sub acute experimental concentrations of phenol were 0,0.18,0.32,0.56,1.00,1.35 mg/L and the exposure time was set up at 4,8,12,16 d.Glutathione peroxidase(GSH-PX),superoxide dismutase(SOD) and glutathione S-transferase(GST) activity in liver were used as an indicator of phenol pollution on toxicological effect of Takifugu rubripes.The results showed that phenol was a drastic toxicant,the 24 h,48 h and 96 h median lethal concentrations of phenol were 17.56 mg/L,15.41 mg/L and 12.09 mg/L respectively,the safe concentration was 1.21 mg/L.Furthermore,the results of sub acute experiment showed that the activities of SOD and GST in the liver tissues of Takifugu rubripes were restrained obviously within 16 d(P<0.05).With the extension of exposure time,GSHPX activity first increased and then showed a decreasing trend,the maximum value from high concentration moved towards low-concentration group gradually.Compared to the control group(0 mg/L),the total SOD activity in each group also first increased and then decreased after a downward trend.In addition to 0.18 mg/L concentration,the other concentrations were significantly different(P<0.05)within 16 d.Basically the concentrations of GST activity decreased gradually with the extension of exposure time.

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