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Position: Home > Articles > Effcets of Insulin-Like Growth Factor 1 on the Proliferation Organic Center Stem Cells at 30 days Growing Period of Antler in Vitro Chinese Agricultural Science Bulletin 2008,24 (2) 1-3

IGF1对生长30d鹿茸的体外培养鹿茸生长中心干细胞的影响

作  者:
冯海华;赵丽红;闭兴明;李光凤;岳占碰;李春义;邓旭明
单  位:
新西兰INVERMAY农业研究中心;吉林大学畜牧兽医学院
关键词:
IGF1;鹿茸;干细胞;梅花鹿
摘  要:
【研究目的】利用细胞培养技术,探讨胰岛素样生长因子(IGF1)对生长30d的鹿茸体外培养不同代数梅花鹿鹿茸生长中心干细胞(鹿茸干细胞)的影响;【方法】原代分离、培养生长30d的鹿茸的梅花鹿鹿茸干细胞,将第2代、5代、8代鹿茸干细胞经含有不同浓度的IGF1(0,1,3,和10nM)作用后,在培养24h后用3H-胸腺嘧啶核苷测定法检测每分钟衰变数(DPM)值;【结果】取材于生长30d鹿茸的干细胞,全部IGF1处理组(1,3,10nM)都显著高于对照组(0nM)(p<0.01)。3个不同浓度IGFⅠ处理组的平均值和最高值都是离体培养2代的细胞最低(分别为2987和3743DPM/mg蛋白),而培养5代的细胞最高(分别为10320和12180DPM/mg蛋白),第8代的细胞又开始下降(分别为8754和11568DPM/mg蛋白);【结论】IGF1能够促进鹿茸干细胞分裂增殖,生长30d鹿茸的干细胞在离体培养,不同培养时期的鹿茸干细胞对IGFⅠ刺激的敏感度不同。
译  名:
Effcets of Insulin-Like Growth Factor 1 on the Proliferation Organic Center Stem Cells at 30 days Growing Period of Antler in Vitro
作  者:
Feng Haihua1,Zhao Lihong1,Bi Xingming1,Li Guangfeng1,Yue Zhanpeng1,Li Chunyi2,Deng Xuming1(1College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062;2AgResearch Limited Invermay Agricultural Centre Puddle Alley,Mosgiel,New Zealand,Private Bag 50034)
关键词:
IGF1,antler,stem cell,Sika Deer
摘  要:
OBJECTIVEUsing cell culture techniques to investigate the effcets of insulin-like growth factor 1(IGF1)on the proliferation of antler organic center stem cells(antler stem cells)from various passages at 30 days growing period of antler in vitro;METHODAntler stem cells were isolated from sika deer antler of growing 30 day and cultured.The second,fifth and eighth population cells were incubated with 0,1,3,and 10 nM IGF1.Thymidine incorporation assay was applied after 24 hours of culture respectively to determine the disintegration per minute(DPM)value;RESULTSThe proliferation of cells from growing 30 day's antler in the experiental group(1,3,10nM)was significantly higher than that of the control group(0nM)(p<0.01).The average and maximal value of the proliferation of cells in the experiental group was lowest in the second population cells(2987 and 3743 DPM/mg respectively),highest in the fifth population cells(10320 and 12180 DPM/mg),and decreased in the eighth population cells again(8754 and 11568 DPM/mg);CONCLUSIONIGF1 can enhance the proliferation of antler stem cells cultured in vitro.The sensitivity of IGF1 on the antler stem cells from various passages at 30 days growing period of antler in vitro.

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