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Position: Home > Articles > Effcets of Insulin-Like Growth Factor 1 on the Proliferation at Different Generation of Antler Organic Center Cells Chinese Agricultural Science Bulletin 2008,24 (4) 1-3

不同培养代数鹿茸生长中心细胞对IGF1刺激的反应

作  者:
梅妹;邓旭明;岳占碰;赵丽红;李春义;冯海华
单  位:
新西兰INVERMAY农业研究中心;吉林大学畜牧兽医学院
关键词:
IGF1;鹿茸;生长中心细胞;梅花鹿
摘  要:
【研究目的】探讨胰岛素样生长因子(IGF1)对生长60d的梅花鹿鹿茸体外培养不同代数鹿茸生长中心细胞的影响。【方法】分离、培养生长60d的梅花鹿鹿茸生长中心细胞,将培养第2代、5代、8代细胞经含有不同浓度的IGF1(0,1,3和10nM)作用,在培养24h后用3H-胸腺嘧啶核苷掺入测定法检测每分钟衰变数(DPM)值。【结果】全部IGF1处理组都显著高于对照组(p<0.01)。不同浓度IGFⅠ处理组的平均值和最高值都是离体培养2代的细胞的DPM最高(分别为31918和39818DPM/mg蛋白),培养5代的细胞(分别为5455和6815DPM/mg蛋白)已大大地下降;到了第8代的(分别为4030和4838DPM/mg蛋白)又有进一步的下降。【结论】IGF1能够促进鹿茸生长中心细胞分裂增殖,生长60d鹿茸的生长中心细胞在离体培养,不同培养时期的鹿茸生长中心细胞对IGFⅠ刺激的敏感度不同。
译  名:
Effcets of Insulin-Like Growth Factor 1 on the Proliferation at Different Generation of Antler Organic Center Cells
作  者:
Mei Mei1, Deng Xuming1, Yue Zhanpeng1, Zhao Lihong1, Li Chunyi2, Feng Haihua1 (1College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, 2Ag Research Limited Invermay Agricultural Centre Puddle Alley, Private Bag 50034, Mosgiel, New Zealand)
关键词:
IGF1, antler, organic center cells, Sika deer
摘  要:
[Objective]To investigate the effects of insulin-like growth factor 1 (IGF1)on the proliferation of antler organic center cells from various passages in vitro at 60 days growing period of antler. [Method]Antler organic center cells were isolated from sika deer antler of growing 60 day and cultured. The second, fifth and eighth population cells were incubated with 0, 1, 3, and 10 nM IGF1. Thymidine incorporation assay was applied after 24 hours of culture respectively to determine the disintegration per minute ( DPM) value.[Results]The proliferation of cells from growing 60 day’s antler in the experimental groupwas significantly higher than that of the control group (p<0.01). The average and maximal DPM value of the proliferation of cells in the experimental group was the highest in the second population cells (31918 and 39818 DPM/mg respectively), decreased in the fifth population cells materially (5455 and 6815 DPM/mg), and decreased in the eighth population cells again (4030 and 4838 DPM/mg). [Conclusion]The results show that IGF1 can enhance the proliferation of antler organic center cells cultured in vitro. The sensitivity of IGF1 on the antler organic center cells from various passages at 60 days growing period of antler in vitro.

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