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当前位置: 首页 > 文章 > 大肠杆菌O157:H7胶体金试纸条研制 食品科学 2010,31 (24) 355-359
Position: Home > Articles > Preparation of Colloidal Gold Strip for the Detection of Escherichia coli O157:H7 FOOD SCIENCE 2010,31 (24) 355-359

大肠杆菌O157:H7胶体金试纸条研制

作  者：
黄岭芳;段霞;陈媛;刘文娟;魏华;赖卫华
单  位：
江西中德生物工程有限公司;南昌大学食品科学与技术国家重点实验室
关键词：
大肠杆菌O157:H7;胶体金;试纸条
摘  要：
采用胶体金标记抗大肠杆菌O157:H7单克隆抗体(鼠源),通过将大肠杆菌O157:H7多克隆抗体和驴抗鼠抗体(二抗)喷涂于硝酸纤维素膜分别作为检测线和质控线,研制大肠杆菌O157:H7胶体金快速检测试纸条。通过优化实验,确定最佳条件为标记量16.8μg/mL、标记pH8.0、封闭剂PEG20000、检测时样品最佳pH7.0～7.5。对26株常见细菌交叉反应结果表明,该试纸条除与鼠伤寒沙门氏菌ATCC13311和金黄色葡萄球菌CMCC26003有轻微交叉反应外,与其他24株菌均无交叉反应。该试纸条灵敏度为104CFU/mL。用该试纸条检测大肠杆菌O157:H7操作简便、快捷、灵敏度高、特异性强。
译  名：
Preparation of Colloidal Gold Strip for the Detection of Escherichia coli O157:H7
作  者：
HUANG Ling-fang1,DUAN Xia1,CHEN Yuan2,LIU Wen-juan2,WEI Hua1,LAI Wei-hua1,(1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2. Jiangxi Zodolabs Biological Engineering Co. Ltd., Nanchang 330029, China)
关键词：
Escherichia coli O157:H7;colloidal gold;strip
摘  要：
Anti-Escherichia coli O157:H7 monoclonal antibody was labeled with colloidal gold to prepare a strip for the detection of E. coli O157: H7. Anti-E. coli O157:H7 polyclonal antibody and donkey anti-mouse IgG were dispensed on the nitrocellulose membrane as the test line and control line, respectively. The optimal conjugation was performed using 16.8μg/mL antibody at the condition of pH 8.0 and blocked with PEG20000. However, the optimal pH range for the detection was 7.0-7.5. Cross-reaction test was conducted by 26 bacteria strains. Results showed no obvious cross-reaction were observed except for the slight cross-reaction from Salmonella typhimurium ATCC13311 and Staphylococcus aureus CMCC26003. Meanwhile, the sensitivity of the developed strip was up to 104 CFU/mL. Moreover, this kind of developed strip was characteristics of simple operation, rapid detection, high sensitivity and specificity.

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