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当前位置: 首页 > 文章 > 微波辅助复合酶法提取脐橙皮中总黄酮的工艺优化 食品工业科技 2017,38 (8) 172-177
Position: Home > Articles > Optimization of extraction process of total flavonoids from navel orange peel by composite enzyme method of microwave assisted Science and Technology of Food Industry 2017,38 (8) 172-177

微波辅助复合酶法提取脐橙皮中总黄酮的工艺优化

作  者：
王晓君;张秀玲;陈少华;杜敏
单  位：
东北农业大学食品学院
关键词：
脐橙皮;总黄酮;微波辅助;复合酶;响应面法
摘  要：
以脐橙皮为原料,采用微波辅助复合酶法对脐橙皮中总黄酮的提取工艺进行优化。选择微波功率、微波时间、微波次数进行微波辅助处理单因素实验,结果表明微波辅助处理的最佳条件为:微波功率240 W,微波时间90 s,微波次数3次;微波辅助处理后,采用Box-Behnken设计方法,探究酶解温度、复合酶添加量、乙醇浓度、酶解时间对橙皮黄酮提取量的影响。结果表明:酶法浸提的最优条件为酶解温度50℃,复合酶添加量5%,乙醇浓度73%,酶解时间为1.5 h。经验证,橙皮黄酮提取量为3.96 g/100 g,与预测值相比,相对误差约1.25%。结果表明实验优化后的工艺参数是可靠的。
译  名：
Optimization of extraction process of total flavonoids from navel orange peel by composite enzyme method of microwave assisted
作  者：
WANG Xiao-jun;ZHANG Xiu-ling;CHEN Shao-hua;DU Min;College of Food Science,Northeast Agricultural University;Collaborative Innovation Center of Forest Resource Development and Utilization;
关键词：
navel orange peel;;flavonoids;;microwave assisted;;compound enzyme;;response surface
摘  要：
Navel orange peel was used as raw materials,optimize the extraction process of total flavonoids from navel orange peel by microwave assisted enzymatic method.Microwave power,microwave time,microwave frequency was chosen to carry out the single factor experiment,the results showed that the optimum microwave power was 240 W,the microwave time was 90 s,the microwave frequency was 3 times.After microwave assisted treatment,Box-Behnken design method was used to study the effect of enzymatic hydrolysis temperature,the amount of compound enzyme,ethanol concentration and the time of enzymatic hydrolysis on the extraction yield of flavonoids. The results showed that the optimal conditions for enzymatic extraction was50 ℃,the amount of the compound enzyme was 5%,the ethanol concentration was 73%,and the time of enzymatic hydrolysis was 1.5 h.The proof test gained extraction of flavonoids was 3.96 g/100 g,and compared with predicted value,the relative error was 1.25%.It suggested that the technical parameters optimized by test were reliable.

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