中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 酶联免疫法检测呕吐毒素的方法研究 食品科学 2007,28 (10) 473-476
Position: Home > Articles > Methodology Study of Indirect Competitive ELISA for Detection of Vomitoxin FOOD SCIENCE 2007,28 (10) 473-476

酶联免疫法检测呕吐毒素的方法研究

作  者：
龚燕;孙秀兰;邵景东
单  位：
江南大学食品科学与技术国家重点实验室;张家港出入境检验检疫局;江苏省微生物研究所
关键词：
酶联免疫法;呕吐毒素;检测
摘  要：
目的:利用抗呕吐毒素的特异性单克隆抗体和包被抗原,建立间接竞争酶联免疫法(ELISA)来检测谷物及其制品中呕吐毒素含量。方法:通过方阵滴定实验确定呕吐毒素最佳抗体浓度和最佳包被抗原浓度,应用酶联免疫测试盒对谷物及其制品中的呕吐毒素进行定量检测的方法学评价,来确立该方法的各项技术指标。结果:方法的最低检测浓度10μg/L;平均RSD为5.8%;回收率平均值为104%;用该方法研制的测试盒在4℃环境下可稳定6个月以上;检测时间小于2h;结论:该方法简便、安全、灵敏度高、重复性好、特异性强、能定性和定量检测谷物及其制品中的呕吐毒素含量。
译  名：
Methodology Study of Indirect Competitive ELISA for Detection of Vomitoxin
作  者：
GONG Yan1,SUN Xiu-lan2,3,SHAO Jing-dong4 (1.Jiangsu Institute of Microbiology, Wuxi 214036, China;2.State Key Laboratory of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China;3.School of Food Science, Southern Yangtze University, Wuxi 214036, China;4.Zhangjiagang Entry-Exit Inspection and Quarantine, Zhangjiagang 215600, China)
关键词：
ELISA;DON;detection
摘  要：
Objective: An indirect competitive ELISA was developed by using anti- DON monoclonal antibody and coating antigen DON-BSA for detection of deoxynivalenol in wheat and corn. Methods: The best dilute concentrations of anti-DON monoclonal antibody and coating antigen were obtained by matrix titration experiment. Methodology of ELISA kit was also studied to ascertain qualifications. Results: The detection limits was 10μg/L. and the linear range of the inhibition curve was 10～500μg/L. The mean coefficients of variation was 5.8%, and mean recovery rate of DON in the samples was 104%. The detection could be finished within 2h. The kit has more than six months stationery phase during 4℃ storage. Conclusion: This ELISA method was verified to be rapid, safe, sensitive, repeatable, specific for DON detection.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46