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Position: Home > Articles > Determination of Furazolidone Metabolite Residue in Pork by Enzyme-linked Immunosorbent Assay MEAT RESEARCH 2011 (12) 29-32

酶联免疫法检测猪肉中呋喃唑酮代谢物残留

作  者:
闵成军;罗晓琴;汪善良;崔廷婷
单  位:
北京勤邦生物技术有限公司;雨润集团
关键词:
呋喃唑酮代谢物;酶联免疫法;猪肉
摘  要:
通过检测猪肉中呋喃唑酮代谢物的残留以评价试剂盒的性能,采用酶联免疫法对猪肉中的呋喃唑酮代谢物残留量进行测定。结果表明:该方法的线性检测范围为0.025~2.025μg/L,最低检测限为0.1μg/kg,样本添加回收率为78.2%~100.3%,变异系数为4.7%~10.3%,与呋喃唑酮的交叉反应率为16.3%,与其他药物的交叉反应率均小于1%,且对实际样本的检测结果与液相色谱-串联质谱法基本一致。该法灵敏准确、重复性好、特异性高,适用于猪肉中呋喃唑酮代谢物残留的检测。
译  名:
Determination of Furazolidone Metabolite Residue in Pork by Enzyme-linked Immunosorbent Assay
作  者:
MIN Cheng-jun1,LUO Xiao-qin2,,WANG Shan-liang2,CUI Ting-ting2(1.Yurun Group Co.Ltd.,Nanjing 210041,China;2.Beijing Kwinbon Biotechnology Co.Ltd.,Beijing 102206,China)
关键词:
furazolidone metabolite(AOZ);enzyme-linked immunosorbent assay(ELISA);pork
摘  要:
An enzyme-linked immunosorbent assay(ELISA) was proposed to determine residual furazolidone metabolite in pork using a commercially available ELISA kit.The results showed that the sensitivity and detection limit of the assay were 0.025-2.025μg/L and 0.1μg/kg,respectively.The recoveries of standard additions of furazolidone metabolite averaged 78.2%-100.3% across three levels(n = 3),and the coefficients of variation were 4.7%-10.3%.The cross-reactivity towards furazolidone was 16.3%;as for other drugs investigated,the cross-reactivity was below 1%.Analytical results obtained using the assay were in substantial accordance with those from liquid chromatographic tandem mass spectrometry(LC-MS/MS) determination.The assay was sensitive,accurate,repeatable,specific and suitable for the determination of furazolidone metabolite residue in pork.

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