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当前位置: 首页 > 文章 > 超声波辅助响应面法提取藜麦多酚及抗氧化性研究 山西农业科学 2016,44 (11) 1708-1714+1737
Position: Home > Articles > Study on Extraction Process by Ultrasonic-assisted Response Surface Method and Antioxidant Activity of Quinoa Polyphenol Journal of Shanxi Agricultural Sciences 2016,44 (11) 1708-1714+1737

超声波辅助响应面法提取藜麦多酚及抗氧化性研究

作  者：
陈树俊;胡洁;王振文;庞震鹏;刘晓娟;徐晓霞;仪鑫;石玥;李乐
单  位：
山西大学生命科学学院
关键词：
藜麦;多酚;响应面;提取;抗氧化性
摘  要：
以山西静乐藜麦为原料,采用超声波辅助响应曲面法对藜麦多酚提取工艺进行优化,并测定其体外抗氧化活性。结果表明,以多酚得率为指标,考虑提取时间、乙醇体积分数、料液比、提取温度4个单因素的影响,在单因素试验的基础上进行响应面实验优化。结果表明,超声波提取藜麦多酚的最佳条件为:提取时间23 min,乙醇体积分数71%,料液比1∶23(g/m L),提取温度52℃;在此条件下,多酚提取量为3.33 mg/g。采用超声波辅助提取藜麦多酚,方法简便,大大缩短了提取时间,增加了提取效率;同时,藜麦多酚提取液具有很强的1,1-二苯基-2-三硝基苯肼(DPPH·)、羟自由基(·OH)清除能力,其半抑制浓度(IC50)分别为1.62,21.27μg/m L,均强于抗坏血酸;此外,藜麦多酚还具有很强的还原能力且其质量浓度与抗氧化活性具有良好的量效关系。说明藜麦富含多酚并且具有良好的抗氧化能力。
译  名：
Study on Extraction Process by Ultrasonic-assisted Response Surface Method and Antioxidant Activity of Quinoa Polyphenol
作  者：
CHEN Shujun;HU Jie;WANG Zhenwen;PANG Zhenpeng;LIU Xiaojuan;XU Xiaoxia;YI Xin;SHI Yue;LI Le;College of Life Sciences,Shanxi University;
关键词：
quinoa;;polyphenol;;response surface;;extraction;;antioxidant activity
摘  要：
Ultrasonic-assisted response surface method was adopted to explore the optimum extraction of polyphenols from Shanxi Jingle quinoa seed, and antioxidant activity in vitro was measured. The paper took polyphenols yield as index, considering the effect on extraction time, ethanol concentration, solid-liquid ratio, extraction temperature four factors. Response surface test was applied based on the single factor experiment. The results showed that the best conditions for ultrasonic extraction of quinoa polyphenols was as follows:extraction time 23 min, ethanol concentration 71%, solid-liquid ratio 1 ∶23(g/m L), extraction temperature 52 ℃, under these conditions, the yield of polyphenols was 3.33 mg/g. The ultrasonic assisted method was used to extract quinoa polyphenols which was simple, greatly shorten the extraction time and increased extraction efficiency. At the same time, the polyphenols extracted from quinoa showed strong radical scavenging activity against 1,1-diphenyl-2-picrylhydrazxyl(DPPH·)and hydroxyl(·OH)which were stronger than ascorbic acid, and half inhibitory concentration(IC50)of them were 1.62, 21.27 μg/m L, respectively. Besides, quinoa polyphenol also had strong reducing power and in a concentration-dependent manner with antioxidant activity. These results illustrated that quinoa was rich in polyphenols and had good antioxidant abilities.

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