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当前位置: 首页 > 文章 > 滤食性鲢、鳙肠含物PCR-DGGE指纹分析 水产学报 2009,33 (6) 82-89
Position: Home > Articles > PCR-DGGE fingerprinting analysis on intestinal contents of filter-feeding fishes (Hypophthalmichthys molirix and Aristichthys nobilis) Journal of Fisheries of China 2009,33 (6) 82-89

滤食性鲢、鳙肠含物PCR-DGGE指纹分析

作  者：
颜庆云;余育和;张堂林;冯伟松;李学梅
单  位：
中国科学院水生生物多样性与保护重点实验室;中国科学院研究生院;中国科学院水生生物研究所
关键词：
鲢;鳙;PCR-变性梯度凝胶电泳;肠含物;武汉东湖
摘  要：
以采自武汉东湖的滤食性鲢、鳙为对象,通过PCR-DGGE并结合序列分析对其肠道微生物及肠含物中残留的食物组分进行了探索研究。在所有个体中都能检测到不同的PCR-DGGE指纹谱带,其中包括肠道细菌在内的原核谱带较多,真核谱带相对较少;分析结果表明针对鲢、鳙肠含物这一特殊生境样品进行PCR-DGGE指纹分析是可行的。PCR-DGGE指纹结构及针对部分特定PCR-DGGE谱带的序列分析显示,从武汉东湖采集的鲢、鳙在食性上存在很大的重叠,并没有像基于常规食性分析文献报道的那样明显不同。基于肠含物DNA来进行鱼类食性分析的方法不受对食物碎片分类鉴定的制约,可同时对多个样品平行进行分析,且不同研究者间的研究结果便于进行比较,以便总结生态学的内在规律。
译  名：
PCR-DGGE fingerprinting analysis on intestinal contents of filter-feeding fishes (Hypophthalmichthys molirix and Aristichthys nobilis)
作  者：
YAN Qing-yun1,YU Yu-he1,ZHANG Tang-lin1,FENG Wei-song1,LI Xue-mei1,2 (1. Key Laboratory of Biodiversity and Conservation of Aquatic Organisms,Institute of Hydrobiology, Chinese Academy of Sciences,Wuhan 430072,China; 2. Graduate School of Chinese Academy of Sciences,Beijing 100039,China)
关键词：
Hypophthalmichthys molirix;Aristichthys nobilis;PCR-DGGE;intestinal contents;Donghu Lake
摘  要：
PCR-DGGE fingerprinting was explored in the present study to analyze intestinal contents of filter-feeding fishes (Hypophthalmichthys molirix and Aristichthys nobilis),which were sampled from the Donghu Lake,Wuhan. Different microbial bands (16S rDNA) and eukaryotic bands (18S rDNA) were detected in all the individuals,and the numbers of 16S rDNA bands were more than that of 18S rDNA bands. Results of the present study suggested that it is possible and useful to apply PCR-DGGE fingerprinting to studying the intestinal contents of filter-feeding fishes. Additionally,PCR-DGGE fingerprinting and sequences analysis of the DGGE bands indicated that intestinal contents composition of silver carp and bighead carp,which had been found to be significantly different in traditional dietary analyses with microscopy identification,was not significantly different. These results suggested that a significant portion of planktonic organisms were fed by the silver carp and bighead carp. In DNA based dietary analyses,multiple samples can be studied simultaneously with a standardized set of procedures,making it practical to perform comparative analyses,and furthermore,this comparison makes it possible to elucidate some objective ecological rules.

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