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当前位置: 首页 > 文章 > 红松多酚与真菌多糖联合清除ABTS自由基活性比较 北京林业大学学报 2016,38 (10) 104-111
Position: Home > Articles > Combined ABTS radical scavenging activity of Pinus koraiensis polyphenols with fungus polysaccharides Journal of Beijing Forestry University 2016,38 (10) 104-111

红松多酚与真菌多糖联合清除ABTS自由基活性比较

作  者：
张乃珣;尹红力;赵鑫;刘冉;于美汇;王振宇
单  位：
东北林业大学林学院;哈尔滨工业大学化工学院食品科学与工程系;中国农业科学院农产品加工研究所主食加工技术研究院;聊城大学农学院
关键词：
真菌多糖;红松多酚;联合;抗氧化活性
摘  要：
本文比较了红松多酚(PKP)与黑木耳多糖(AAP)和灵芝孢子多糖(GLP)单一、联合使用清除ABTS自由基活性的作用。采用Chou-Talaly联合指数方法分析ABTS自由基清除实验结果,并对单一和联合使用的清除效果、联合指数(CI)、等效应图和剂量减少指数(DRI)进行分析评价。结果显示:单独使用红松多酚、黑木耳多糖、灵芝孢子多糖的IC50值分别为72.01μg/m L、2.49 mg/m L、0.93 mg/m L,联合使用红松多酚和黑木耳多糖与红松多酚和灵芝孢子多糖(质量比为1∶3)后,IC50值分别为185.20和237.83μg/m L。联合指数分析表明,从清除率10%到97%,红松多酚和黑木耳多糖的联合指数都小于1;等效应图显示联合使用红松多酚和黑木耳多糖使用剂量降低。剂量减少指数分析显示,从清除率5%到97%,红松多酚和黑木耳多糖的剂量减少指数都大于1,以上结果红松多酚和灵芝孢子多糖均表现不一致。说明红松多酚和黑木耳多糖之间存在协同清除ABTS自由基效应,但红松多酚和灵芝孢子多糖不存在。
译  名：
Combined ABTS radical scavenging activity of Pinus koraiensis polyphenols with fungus polysaccharides
作  者：
ZHANG Nai-xun;YIN Hong-li;ZHAO Xin;LIU Ran;YU Mei-hui;WANG Zhen-yu;College of Forestry,Northeast Forestry University;Institute of Food Science and Technology CAAS/Institute of Staple Food Processing Technology;Agricultural College,Liaocheng University;Department of Food Science and Engineering,Harbin Institute of Technology;
关键词：
fungus polysaccharides;;Pinus koraiensis polyphenols;;combination;;antioxidant activity
摘  要：
We compared the ABTS radical scavenging activity of Pinus korsiensis polyphenols( PKP) alone or combined with Auricularia auricular polysaccharides( AAP) and Ganoderma lucidum spore polysaccharides( GLP). ABTS radical scavenging experiments and Chou-Talalay combination index( CI)method were used to evaluate the scavenging effects of PKP with AAP and GLP individually as well as in combination,and combination index( CI),isobologram and dose-reduction index( DRI) were worked out subsequently. It showed that IC50 values of PKP,AAP and GLP were 72. 01 μg / mL,2. 49 mg / mL and 0. 93 mg / mL,respectively,while IC50 values of the combination( mass ratio 1∶ 3) were 185. 20 and237. 83 μg / mL,respectively. CI analysis indicated that the clearance rate was 10%-97%,and CI of PKP with AAP was less than 1. Dose of PKP with AAP was reduced based on isobologram. DRI analysis demonstrated that the clearance rate was 5%-97%,and DRI of PKP with AAP was greater than 1. Theresults of PKP with GLP were inconsistent. Our study suggests that the combination of PKP and AAP has significantly improved the ABTS radical scavenging activity,compared with using them individually.

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