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Position: Home > Articles > Cloningand Quantitative Expression of a β-Glucosidase Gene from Inonotus obliquus Acta Edulis Fungi 2015,22 (3) 1-6

桦褐孔菌β-葡萄糖苷酶基因的克隆与定量表达分析

作  者:
隋飞飞;陈艳秋
单  位:
延边大学农学院
关键词:
桦褐孔菌;内切葡聚糖酶;菌核
摘  要:
利用反转录PCR(reverse transcription PCR,RT-PCR)和cDNA末端快速扩增(rapid-amplification of cDNA ends,RACE)技术从桦褐孔菌(Inonotus obliquus)菌核中克隆了β-葡萄糖苷酶基因的全长DNA序列,命名为IO-BGL。该DNA序列全长3382bp,其中开放阅读框(ORF)长度为2583bp,含有13个内含子和14个外显子,编码860个氨基酸,相对分子量为93.4kD,等电点为5.57,编码的氨基酸序列与地中海嗜蓝孢孔菌(Fomitiporia mediterranea)相似性最高(85%)。采用实时荧光定量PCR技术研究IO-BGL基因在菌核发育过程中的表达量变化,结果表明,IO-BGL基因在菌核的发育过程中,表达量呈先上升后下降趋势。
译  名:
Cloningand Quantitative Expression of a β-Glucosidase Gene from Inonotus obliquus
作  者:
SUI Feifei;CHEN Yanqiu;College of Agriculture,Yanbian University;
关键词:
Inonotus obliquus;;β-glucosidase;;sclerotium
摘  要:
The full-length DNA sequence of aβ-glucosidase gene(IO-BGL)was cloned from Inonotus obliquus sclerotia by RT-PCR and RACE.The gene had a molecular mass of 93.4kD,apI value of 5.57,and consisted of 3382 bp,13introns and 14 exons,and an open reading frame(ORF)of 2583 bp that encoded a protein of 860 amino acids.The putative amino acid sequence of IO-BGL was 85%similar to aβ-glucosidase from Fomitiporia mediterranea.Real time quantitative PCR revealed that IO-BGL expression increased during the first 170 days of sclerotium development and then decreased.

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