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当前位置: 首页 > 文章 > 陕西省猕猴桃黑斑病病原菌鉴定 植物病理学报 2020 (1) 112-116
Position: Home > Articles > Identification of the pathogen causing black spot on kiwifruit in Shaanxi Province Acta Phytopathologica Sinica 2020 (1) 112-116

陕西省猕猴桃黑斑病病原菌鉴定

作  者：
付博;王家哲;任平;李英梅;赵杰;金平涛;张锋
单  位：
陕西省生物农业研究所陕西省植物线虫学重点实验室;陕西省酶工程技术研究中心;西北农林科技大学植物保护学院;陕西省周至县植保植检站
摘  要：
‘翠香猕猴桃’属美味猕猴桃品系,具有早熟优质、味道香甜等优点,是陕西省周至县、鄠邑区、眉县等猕猴桃主产区特有的主栽品种[1]。2013年起,陕西周至‘翠香猕猴桃’果园连年发生黑斑病,平均病园率达85.8%,病果率达48.5%以上[2]。该病在果实生长后期呈现黑色病斑症状并在低温贮藏期引起果实腐烂,严重制约了猕猴桃产业的发展。‘翠香猕猴桃’黑斑病病原菌种类、形成机理尚不明确。本研究针对该病发生情况进行田间调查和病原菌分离,通过回接试验
译  名：
Identification of the pathogen causing black spot on kiwifruit in Shaanxi Province
作  者：
FU Bo;WANG Jia-zhe;REN Ping;LI Ying-mei;ZHAO Jie;JIN Ping-tao;ZHANG Feng;Bio-Agriculture Institute of Shaanxi,Shaanxi Key Laboratory of Plant Nematology;Enzyme Engineering Research Center of Shaanxi Province;Northwest A&F University,College of Plant Protection;Zhouzhi Plant Protection and Quarantine Station in Shaanxi Province;
关键词：
Kiwifruit;;black spot;;pathogenic fungi;;Diaporthe eres
摘  要：
To confirm the causal pathogen and damage degree of a new disease of kiwifruit-black spot in Shaanxi Province, a field investigation was conducted in Zhouzhi County and Huyi District in 2018. The pathogen was isolated from the diseased fruits and identified based on morphological characteristics, pathogenicity testing, sequence alignment and phylogenic tree analysis. The results showed that the average incidence of black spot was 65%, and the early fruiting rate was 33%. Fruits infected with black spot disease were soft-rotted after being stored for two months under low temperature conditions. The pathogenic fungi were isolated from the under skin of Cuixiang kiwifruits and the strains could produce two types of conidia, including oval α-type conidia and curved β-type conidia. Through sequences alignment of 18 S rRNA, ITS, reference gene(EF1-α) and phylogenetic tree clustering analysis, it was identified for the first time that the causal pathogen causing black spot disease of Cuixiang kiwifruit in Shaanxi Province was Diaporthe eres.

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