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Position: Home > Articles > 16S rDNA-based Identification of Bifidobacterium in Foods FOOD SCIENCE 2009,30 (18) 359-361

应用16S rDNA鉴定食品中的双歧杆菌

作  者:
吴拥军;王嘉福
单  位:
贵州省农业生物工程重点实验室;贵州大学生命科学学院
关键词:
双歧杆菌;PCR;鉴定;16SrDNA
摘  要:
依据双歧杆菌16SrDNA保守序列设计属特异性引物,利用聚合酶链式反应(PCR)扩增技术对食品中分离物基因组扩增,进行双歧杆菌属的分子鉴定。建立了相应快速、可靠的双歧杆菌基因组DNA的提取方法和PCR扩增体系。这种快速、可靠的鉴定方法能够有效的检测食品及微生态制剂中的双歧杆菌。
译  名:
16S rDNA-based Identification of Bifidobacterium in Foods
作  者:
WU Yong-jun1,2,WANG Jia-fu1 (1. College of Life Sciences, Guizhou University, Guiyang 550025, China; 2. Guizhou Key Laboratory of Agricultural-Bioengineering, Guiyang 550025, China)
关键词:
Bifidobateriim;PCR;identification;16S rDNA
摘  要:
According to the conservative sequence of16S rDNA from Bifidobacterium genus, two pairs of specific primers were developed, named BifS11 and BifS12, for molecular biological identification of strains of the genus Bifidobacterium by PCR. The combination of ultrasonic treatment and proteinase K-catalyzed hydrolysis resulted in higher purity of genomic DNA than other three methods, and high specificity PCR assays were achieved using the two pairs of primers. The PCR array is rapid and reliable, can provide an efficient detection of Bifidobacterium in foods and probiotics.

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