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Position: Home > Articles > Screening of Bacteriocin-producing Bif idobacterium Strain and Culture Coditions for Bacteriocin Secretion FOOD SCIENCE 2013,34 (23) 170-175

产细菌素双歧杆菌的筛选及其分泌条件研究

作  者:
尚楠;王洋;任发政;李平兰
单  位:
中国农业大学食品科学与营养工程学院
关键词:
双歧杆菌;筛选;细菌素;分泌特性
摘  要:
以32株我国广西巴马长寿老人源双歧杆菌为材料,以植物乳杆菌PL-2为指示菌,经筛选获得1株具有明显抗菌效果的双歧杆菌L-SN。排除有机酸、H2O2等干扰因素,经硫酸铵沉淀粗提,蛋白酶敏感性实验后确定其产生的抗菌物质为细菌素。通过形态学、生理生化以及16S rRNA序列分析鉴定菌株L-SN为动物双歧杆菌乳亚种(Bifidobacterium animalis subsp.lactis L-SN)。进一步研究菌株L-SN产细菌素的生物学特性,确定其最佳分泌细菌素条件为起始pH6.5、培养温度30℃、培养时间28h。在此基础上,考察菌株L-SN所产细菌素的抗菌谱,发现其具有广谱的抗菌效果,尤其对金黄色葡萄球菌、单增李斯特氏菌等致病菌有显著的作用,显示出该双歧杆菌的潜在开发应用前景。
译  名:
Screening of Bacteriocin-producing Bif idobacterium Strain and Culture Coditions for Bacteriocin Secretion
作  者:
SHANG Nan;WANG Yang;REN Fa-zheng;LI Ping-lan;College of Food Science and Nutritional Engineering,China Agricultural University;
关键词:
Bif idobacterium;;screening;;bacteriocin;;secretion characteristics
摘  要:
Bif idobacterium L-SN capable of producing an antimicrobial substance that was effective against Lactobacillus plantarum PL-2 was screened from 32 strains of Bifi dobacterium isolated from the feces of centenarians in Bama county of Guangxi region. By eliminating the effects of organic acid and H 2 O 2, we confi rmed the antimicrobial role because of bacteriocin. Based on morphological, physiological and biochemical characteristics and 16S rRNA sequence analysis, the strain L-SN was identif i ed as Bifi dobacterium animalis subsp. lactis. We investigated the secretion characteristics of Bif idobacterium animalis L-SN and established the optimum incubation conditions for producing bacteriocin: 28 h of culture at 30 ℃ and initial medium pH 6.5. The bacteriocin had a wide spectrum of antibacterial activities against Gram-positive and Gram-negative bacteria, especially against Staphylococcus aureus, Escherichia coli and Listeria monocytogenes showing a potential application prospect.

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