中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 猫初级视皮层BDNF表达年龄相关性差异研究 安徽科技学院学报 2015 (1) 9-13
Position: Home > Articles > Study on Age- related Morphological Changes of BDNF Expression in the Primary Visual Cortex of Cat Journal of Anhui Science and Technology University 2015 (1) 9-13

猫初级视皮层BDNF表达年龄相关性差异研究

作  者：
童传旺;季慕寅;许月明;曹侃
单  位：
芜湖职业技术学院生物工程学院
关键词：
BDNF;猫;初级视皮层;免疫组织化学;年龄相关性;视觉
摘  要：
通过揭示脑源性神经营养因子(BDNF)在青年猫与老年猫初级视皮层(Primary visual cortex)中表达情况,特别是探讨猫视皮层BDNF表达的年龄差异性及可能产生的突触可塑性方面的影响,揭示视觉衰退机制的影响因素及机理。本实验采用免疫组织化学ABC法标记猫初级视皮层各层BDNF免疫阳性细胞。免疫染色切片置于Olympus光学显微镜下观察采集图像,Image-Pro Express 6.0软件处理BDNF免疫阳性细胞计数及免疫阳性反应强度测量,所得相关数据借助SPSS 13.0统计软件分析处理。结果显示,BDNF在青年组及老年组猫视皮层各层中均有表达;与青年组相比,老年组猫初级视皮层各层中BDNF阳性细胞密度显著下降(P<0.01),其免疫阳性反应强度亦呈显著下降(P<0.01)。结果提示,猫初级视皮层中BDNF表达水平存在增龄性下降,提示脑源性神经营养因子活动减弱引起的突触可塑性的改变可能是引起视觉功能衰退的重要原因之一。
译  名：
Study on Age- related Morphological Changes of BDNF Expression in the Primary Visual Cortex of Cat
作  者：
TONG Chuan-wang;JI Mu-yin;XU Yue-ming;CAO Kan;College of Biological Engineering,Wuhu Institute of Technology;
关键词：
BDNF;;Cats;;Primary visual cortex;;Immunohistochemistry;;Age-related;;Vision
摘  要：
This study compared the expression of brain- derived neurotrophic factor( BDNF) in the primary visual cortex between young adult and old cats,especially the age- related difference of BDNF expression in cat's visual cortex and the possible influence on synaptic plasticity,trying to probe into the influencing factors and neural mechanisms that mediate aging- related visual function declines. BDNF- IR cells in each cortical layer were labeled using immunohistochemical techniques. Staining sections were observed under Olympus optical microscope and photographed with a digital camera. The density of BDNF- IR cells and immunoreactive intensity were measured with Image- Pro Express 6. 0 software. BDNF proteins were widely expressed in all cortical layers of young adult and old cats. Compared with the young adult cats,the density of BDNF- IR cells in each cortical layer of old cats was significantly decreased( P < 0. 01). Furthermore,BDNF- IR intensity in the old group of cats,as indicated by the mean optical density,was significantly weaker than that in the young adult group( P < 0.01). Aging results in a significantly decreased expression of BDNF proteins. This indicates that changes of synaptic plasticity,which are caused by the decreased activity of BDNF,might be one of the important factors that cause a compromised inhibition in the central visual nucleus and a declined visual function in senescent individuals.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46