中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 连翘酯苷A抑制LPS诱导的鸡法氏囊中炎症因子表达 东北农业大学学报 2013,44 (12) 58-63
Position: Home > Articles > Forsythiaside A inhibit inflammation factor expression on bursa of Fabricius in chicken stimulated by Lipopolysaccharide Journal of Northeast Agricultural University 2013,44 (12) 58-63

连翘酯苷A抑制LPS诱导的鸡法氏囊中炎症因子表达

作  者：
程广东;闫清波;陈俭清;盛尊来;赵玉莲;韩强;赵玉林;黄全勇;周永辉
单  位：
东北农业大学生命科学学院;东北农业大学动物医学学院
关键词：
连翘酯苷A;LPS;法氏囊;炎症因子;基因表达
摘  要：
为探究连翘酯苷A抑制LPS诱导鸡法氏囊中炎性因子的表达,将40只新生雏鸡随机分成四组,分别为空白组、LPS组、连翘酯苷(30和60 mg·kg-1)治疗组,每组10只。所有鸡正常饲喂至15日龄时连翘酯苷治疗组开始灌胃给药,每天1次连续7 d后,除空白组外,其余各组鸡均翅下静脉注射200μg·kg-1LPS,对各组鸡法氏囊制作石蜡切片,并采用实时荧光定量PCR和ELISA方法,检测IL-8、IL-17和IL-10 mRNA表达水平及IL-17蛋白含量。结果表明,LPS组法氏囊IL-8、IL-17和IL-10 mRNA表达水平增加,IL-17蛋白含量上升,病理变化明显;而连翘酯苷治疗组IL-8、IL-17和IL-10 mRNA表达水平下降,IL-17蛋白含量下降,病理变化减轻。结果显示,LPS能升高鸡法氏囊炎症因子mRNA和蛋白表达水平,而连翘酯苷A与LPS作用相反,进而推测连翘酯苷A可抑制由LPS诱发的炎症因子IL-8、IL-17及IL-10的转录,并干扰IL-17蛋白的翻译,进而影响NF-κB通路,引起炎症因子的释放,最终发挥抗炎功效。
译  名：
Forsythiaside A inhibit inflammation factor expression on bursa of Fabricius in chicken stimulated by Lipopolysaccharide
作  者：
CHENG Guangdong;YAN Qingbo;CHEN Jianqing;SHENG Zunlai;ZHAO Yulian;HAN Qiang;ZHAO Yulin;HUANG Quanyong;ZHOU Yonghui;LI Yanhua;School of Veterinary Medicine,Northeast Agricultural University;School of Life Science,Jiamusi University;School of Life Science,Northeast Agricultural University;
关键词：
Forsythiaside A;;Lipopolysaccharide;;bursa of Fabricius;;inflammation factor;;gene expression
摘  要：
In order to study the inflammation factor expression that forsythiaside A inhibited LPS on bursa of Fabricius of chicken, forty chicken were randomly divided into control group, LPS group and forsythiaside A(30, 60 mg· kg- 1) therapy group,10 chicken in each group. All chicken were administerated with vehicle, until chicken were 15 d old and were oral administrated by forsythiaside A(30, 60 mg· kg-1) once a day for 7 d on forsythiaside A therapy group. when chicken were 21 d old, after 2 h, when forsythiaside A was oral administrated for last time, and chicken on other three groups except the control group wereinjected intravenously with LPS at 200 μg · kg- 1, and 2 h later, then bursa of Fabricius of chicken were separated for paraffin section and the expression level of mRNA and protein of inflammation factor of bursa of Fabricius in chicken was tested by Real-time PCR and ELISA methods. The results showed that IL-8, IL-17 and IL- 10 mRNA expression and IL- 17 protein content increase and pathological changes appear obviously on bursa of Fabricius of chicken in LPS group, while it is opposite in forsythiaside A(30, 60 mg· kg-1)therapy group, and the above results showed that LPS could increase inflammation factor mRNA expression and protein content, while Forsythiaside A decrease inflammation factor mRNA expression and protein content. It was speculated that Forsythiaside A could inhibit transcription of IL-8, IL-17 and IL-10, interfere with the translation of IL- 17, further affected NF- ?B signaling pathway, and led to inflammation factor release, at last function anti-inflammatory efficacy.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46