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Position: Home > Articles > Effects of shikonion the proliferation and apoptosis of hepatoma carcinoma cells Heilongjiang Animal Science and Veterinary Medicine 2010 (7) 16-18

紫草素对肝癌细胞增殖和凋亡的影响

作  者:
赵瑞杰;李引乾;王会;王广彬;关伟军
单  位:
中国农业科学院北京畜牧兽医研究所;中国农业科学院北京畜牧兽医研究所西北农林科技大学动物医学学院;西北农林科技大学动物科技学院;辽宁医学院食品科学与工程学院
关键词:
紫草素;肝癌细胞;凋亡
摘  要:
为了研究紫草素对肝癌细胞增殖和凋亡的影响,试验以SMMC-7721为研究对象,利用倒置显微镜观察细胞的形态变化,通过体外细胞毒性试验(MTT)比色法和流式细胞仪检测紫草素处理前后对肝癌细胞株SMMC-7721增殖和诱导细胞凋亡的作用。结果表明:在紫草素作用下,SMMC-7721形态发生改变,细胞增殖受到抑制,细胞凋亡率为(19.00±1.31)%和(23.04±1.25)%。说明紫草素对肝癌细胞株SMMC-7721具有明显的凋亡诱导作用,为抗肿瘤药物的研究提供了理论基础。
译  名:
Effects of shikonion the proliferation and apoptosis of hepatoma carcinoma cells
作  者:
ZHAO Rui-jie1,LI Yin-qian1,WANG Hui2,WANG Guang-bin2,GUAN Wei-jun2(1.College of Veterinary Medicine,Northwest Agriculture and Forestry University,Yangling 712100,China;2.China Institue of Animal Sicences,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100193,China)
关键词:
Shikonion;hepatoma carcinoma cells;apoptosis
摘  要:
In this research,SMMC-7721,a hepatoma carcinoma cell line was used to evaluate the inhibitory and apoptotic effects of shikonion on tumourigenesis,the morphological changes upon treatment were observed with phase contrast microscopy,cytotoxicity was analysed with MTT assay,and proliferative and apoptotic effects were detected with flow cytometry.The results suggested that upon treatment with shikonion the cells became irregular in morphology,were inhibited in growth and division,and exhibited apoptotic rates of(19.00±1.31)% and(23.04±1.25)%.This study proved that shikonion did have significant pro-apoptotic effects on SMMC-7721 line,and hence provided a theoretical basis for future development of prospective anti-tumor drugs.

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