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Position: Home > Articles > Isolation of a Cd-resistant Bacterium and Optimization of Its Bio-accumulation Condition Soils 2016 (3) 615-620

一株耐镉细菌的分离鉴定及其吸附条件的优化

作  者：
沈秋悦;曹志强;朱月芳;施维林
单  位：
苏州科技学院环境科学与工程学院苏州市环境监测中心;苏州科技学院环境科学与工程学院
关键词：
耐镉细菌;分离鉴定;生物吸附;吸附机理
摘  要：
从镉污染场地筛选分离得到一株耐镉细菌F7,经过形态学观察以及16S r DNA同源性对比分析,鉴定菌株F7属于芽孢杆菌(Bacillus),最大耐Cd~(2+)浓度为50 mg/L。实验研究了Cd~(2+)初始浓度、pH及投菌量对菌株吸附Cd~(2+)的影响,利用FTIR探究菌株吸附的机理。结果表明:菌株F7在Cd~(2+)初始浓度为1.0 mg/L、投菌量为1.0 g/L、pH为6.1时,对Cd~(2+)的吸附率达到93.9%;吸附符合Langumir模型,最大吸附容量为1.83 mg/g。对比分析吸附前后的红外光谱图,发现菌株F7表面的官能团羟基、胺基、烷基、蛋白酰胺Ⅱ带及磷酸基团在吸附过程中起主要作用。
译  名：
Isolation of a Cd-resistant Bacterium and Optimization of Its Bio-accumulation Condition
作  者：
SHEN Qiuyue;CAO Zhiqiang;ZHU Yuefang;SHI Weilin;School of Environmental Science and Engineering, Suzhou University of Science and Technology;Environmental Monitoring Center of Suzhou;
关键词：
Cd-resistant bacterium;;Isolation and identification;;Biosorption;;Adsorption mechanism
摘  要：
A native bacterial strain, named F7, with strong Cd resistance ability was isolated from Cd-contaminated soil. According to its morphological and biochemical characteristics and 16 S r DNA sequences analysis, it was identified as Bacillus. The bacterial strain F7 could grow on the beef extract peptone medium that concentrated Cd~(2+), which can reach to 50 mg/L. The effects of initial Cd~(2+) concentration, p H and biosorbent dosage on Cd~(2+) adsorption efficiency were studied. And the adsorption mechanism of bacterial strain was explored by FTIR. The experimental results showed that the Cd~(2+) adsorption efficiency achieved 93.9% when initial Cd~(2+) concentration was 1.0 mg/L, biosorbent dosage was 1.0 g/L, and p H was 6.1. The biosorption equilibrium conformed to the Langumir equations and the maximum adsorption capacity was 1.83 mg/L. The functional groups on the cell wall of bacterial strain F7 before and after adsorption process were observed by FTIR, the results indicated that the main functional groups participated in Cd adsorption were-OH,-NH_2,-CH_3, Protein amide Ⅱ and Phosphate group.

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