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当前位置: 首页 > 文章 > 1株耐Cd细菌的分离、鉴定及其吸附特性研究 河南农业科学 2017,46 (5) 71-76+83
Position: Home > Articles > Isolation,Identification and Adsorption Characteristics of a Cd-tolerant Bacterium Journal of Henan Agricultural Sciences 2017,46 (5) 71-76+83

1株耐Cd细菌的分离、鉴定及其吸附特性研究

作  者：
徐淑霞;杜文涛;王晓雅;张继冉;张世敏;吴坤
单  位：
河南农业大学生命科学学院/农业部农业微生物酶工程重点实验室
关键词：
耐镉细菌;路德维希肠杆菌;生物吸附;电镜观察;红外光谱分析
摘  要：
从长期受农药污染的土壤中分离筛选到1株可耐受重金属Cd的菌株,经形态观察、生理生化特性、16S rRNA基因序列和系统发育分析确定该菌株为路德维希肠杆菌(Enterobacter ludwigii),命名为EM1,为Cd污染水体及土壤的修复提供微生物菌株。在无机盐培养基中菌株EM1对Cd~(2+)的最适耐受质量浓度为50 mg/L,吸附Cd~(2+)的最适生长温度为35℃,最适pH值为6.0,在此条件下,其对Cd~(2+)的吸附率最大,为92%。电镜扫描结果表明,在吸附Cd~(2+)的过程中菌体表面分泌大量的胞外多聚物且菌体形态发生变化。红外光谱分析显示,Cd~(2+)胁迫条件下,菌体细胞壁结构并未发生变化,菌株吸附Cd~(2+)的过程主要与菌体表面的羟基、N-H、C-H、多糖中的C-O-C及酰胺基团有关。
译  名：
Isolation,Identification and Adsorption Characteristics of a Cd-tolerant Bacterium
作  者：
XU Shuxia;DU Wentao;WANG Xiaoya;ZHANG Jiran;ZHANG Shimin;WU Kun;College of Life Sciences,Henan Agricultural University/Key Laboratory of Agricultural Microbial Enzyme Engineering of Ministry of Agriculture;
关键词：
Cd-tolerant bacterium;;Enterobacter ludwigii;;bioadsorption;;electron microscopic observation;;infrared spectroscopy analysis
摘  要：
A Cd-tolerant strain was isolated from the pesticide polluted soil,on the basis of morphological observation,physiological and biochemical characteristics,16 S rRNA gene sequence and phylogenetic analysis,the strain was identified as Enterobacter ludwigii,and named as EM1,so as to provide strain for remediation of Cd polluted water and soil. The optimal Cd~(2+)concentration of strain EM1 against Cd~(2+)was50 mg/L,the optimum growth temperature of strain EM1 against Cd~(2+)was 35 ℃,and the optimum pH value of strain EM1 against Cd~(2+)was about 6. 0. Under this condition,the Cd~(2+)adsorption rate of EM1 was the highest,reached 92%. The SEM results showed that in the process of adsorption of Cd~(2+),the morphology of the strains was changed,and a large number of extracellular polymeric substances were secreted on the strain surface. Infrared scanning analysis showed that under the Cd~(2+)stress condition,the cell walls structure had not changed,and hydroxy,N-H,C-H,C-O-C and aminoacyl were confirmed to be the key functional groups for the strain to adsorb Cd~(2+).

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