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Position: Home > Articles > Studies on the Rapid Detection of Foot and Mouth Disease Virus Using One Step RT PCR and Nested PCR China Animal Health Inspection 1997 (2)

运用RT-PCR一步法和Nested-CR法快速检测FMDV的研究

作  者:
蒋正军;尹燕博;龚振华;马洪超;孙淑芳;蔡丽娟;郭福生;李葳;吴时友;黄运生;林庆燕;丁有胜;骆德海;陈义民
单  位:
农业部动物检疫所;深圳动植物检疫局
摘  要:
将O、A型FMDVRNA分离纯化,用RT-PCR法扩增其聚合酶编码基因,可检测到A型10-6倍稀释、O型10-4倍稀释乳鼠肌肉毒。Nested-PCR扩增该基因内部片段,进一步说明该方法准确可靠。RT-PCR一步完成使整个检测时间不超过12小时,可在本病的诊断和检疫中推广此法
译  名:
Studies on the Rapid Detection of Foot and Mouth Disease Virus Using One Step RT PCR and Nested PCR
作  者:
Jiang Zheng jun,Yin Yan bo,Gong Zhen hua,Ma Hong chao,Sun Shu fang, Cai Li juan,Guo Fu sheng,Li Wei,Wu Shi you (Animal Quarantine Institnte,Ministry of Agriculture,Qingdao 266032) Huang Yun sheng,Lin Qing yan,Ding You sheng,Luo De hai,Che
摘  要:
An RT PCR was developed to amplify the RNAs of Types A and O FMDV.The sensitivity reached 10 -6 for Type A and 10 -4 for Type O.Amplification of the inner fragments of the gene confirmed the reliabilty for the detection of FMDV.The completion of the whole RT PCR was no more than 12 hours .It is suggested that RT PCR be applied for the import quarantine diagnosis and detection of FMD.

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