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Position: Home > Articles > PCR Detection of phyA Transgenic Maize Journal of Henan Agricultural Sciences 2011,40 (10) 41-43

转植酸酶基因(phyA)玉米的PCR检测

作  者:
赵丽娜;关淑艳;王丕武;楚海娇;季丽丽
单  位:
吉林农业大学生物技术中心
关键词:
花粉管通道法;玉米;PCR检测;植酸酶基因
摘  要:
采用CTAB法和SDS法对通过花粉管通道法导入植酸酶基因(phyA)的玉米植株,进行基因组提取并做了对比,认为SDS法效果更好,进而进行了PCR检测,并对PCR反应体系及反应条件进行优化,得出最适反应条件为57℃、模板含量1μL,检测得到了转基因阳性植株,初步证明外源目的基因已整合到玉米基因组中。
译  名:
PCR Detection of phyA Transgenic Maize
作  者:
ZHAO Li-na,GUAN Shu-yan,WANG Pi-wu,CHU Hai-jiao,JI Li-li (Biotechnology Center of Jilin Agricultural University,Changchun 130118,China)
关键词:
Pollen-tube pathway;Maize;PCR detection;phyA
摘  要:
The CTAB method and SDS method were separately used to extract genome from phyA transgenic maize obtained by the pollen-tube pathway.By comparison,the SDS method obtained a better result.The genome was analyzed by PCR method,and the reaction system and reaction conditions were optimized.The optimal reaction condition was 57℃ with 1μL DNA as template.The result of PCR detection showed that positive transgenic plants were obtained and the foreign phyA gene was integrated into maize genome.

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