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Position: Home > Articles > Construction of a Versatile Escherichia coli-Bacillus subtilis Shuttle Vector and Its Application in Protease Gene Expression Biotechnology Bulletin 2013 (9) 146-150

大肠杆菌-枯草杆菌穿梭载体的构建及其在蛋白酶基因表达中的应用

作  者:
杨键;游志庆;麦志茂;李洁;田新朋;张偲
单  位:
中国科学院南海海洋研究所
关键词:
穿梭载体;P43启动子;枯草芽孢杆菌表达系统;蛋白酶;分泌表达
摘  要:
利用基因重组技术,以枯草杆菌表达载体pWB980为骨架,插入大肠杆菌的克隆载体pUC18中的抗性基因和复制子,构建穿梭质粒pY980coli。将海洋细菌蛋白酶基因hspa插入pY980coli载体P43启动子下游,导入枯草杆菌WB600中,可实现活性表达。
译  名:
Construction of a Versatile Escherichia coli-Bacillus subtilis Shuttle Vector and Its Application in Protease Gene Expression
作  者:
Yang Jian;You Zhiqing;Mai Zhimao;Li Jie;Tian Xinpeng;Zhang Si;Key Laboratory of Marine Bio-resources Sustainable Utilization,South China Sea Institute of Oceanology,Chinese Academy of Sciences;Graduate University of the Chinese Academy of Sciences;
关键词:
Shuttle vector P43 promoter Bacillus subtilisexpression system Protease Secretory expression
摘  要:
A versatile Escherichia coli-Bacillus subtilisshuttle vector pY980coli was constructed by genetic recombination.Resistance gene and replicon of pUC18,a cloning vector forEscherichia coli,were integtated into theBacillus subtilisexpression plasmid pWB980.Protease genehspafrom a marine bacterium was then inserted under P43 promoter of pY980coli,and the recombinant vector was transformed intoBacillus subtilisWB600.Protease activity was detected in the fermentation broth.

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