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当前位置: 首页 > 文章 > 成熟液中不同添加物对猪卵母细胞体外成熟及孤雌胚胎发育的影响 湖北农业科学 2009,48 (10) 2502-2505
Position: Home > Articles > Effect of Different Additive of Maturation Media on Maturation of Porcine Oocytes and Development of Parthenogenetic Embryoes Hubei Agricultural Sciences 2009,48 (10) 2502-2505

成熟液中不同添加物对猪卵母细胞体外成熟及孤雌胚胎发育的影响

作  者：
张立苹;王亚刚;李莉;刘西梅;乔宪凤;许生成
单  位：
青海大学农牧学院;湖北省农业科学院畜牧兽医研究所/动物胚胎工程与分子育种湖北省重点实验室
关键词：
猪卵母细胞;体外成熟;孤雌胚胎
摘  要：
试验研究了在成熟液中分别添加0.1%PVA、10%FBS和不同浓度的EGF(20、30、40、50 ng·mL-1)对猪卵母细胞体外成熟的影响,以确立猪卵母细胞体外成熟的最佳条件。结果表明,在成熟液中添加0.1%PVA和10%FBS后,对猪卵母细胞的成熟率影响无显著差异(P>0.05),对猪卵母细胞孤雌激活后36 h的卵裂率和第6天的桑葚胚率无显著差异(P>0.05);添加不同浓度的EGF对猪卵母细胞体外成熟率无显著差异(P>0.05);但是添加30和40 ng·mL-1 EGF组在36 h的卵裂率要极显著地高于添加20和50ng·mL-1 EGF的两组(P<0.01);而分别添加30、40和50 ng·mL-1 EGF 3组处理在第6天的桑葚胚率无显著差异,且极显著地高于添加20 ng·mL-1 EGF组的(P<0.01)。所以,在成熟液中添加0.1%PVA可以替代10%FBS,尤以添加30 ng·mL-1 EGF组对猪卵母细胞的体外成熟效果较好。
译  名：
Effect of Different Additive of Maturation Media on Maturation of Porcine Oocytes and Development of Parthenogenetic Embryoes
作  者：
ZHANG Li-ping1,WANG Ya-gang2,LI Li2,LIU Xi-mei2,QIAO Xian-feng2,XU Sheng-cheng1(1.College of Animal Husbandry,Qinghai University,Xining 810016,China;2.Institute of Animal and Veterinary Science,Hubei Academy of Agricultural Science / Key Lab of Animal Embryo Engineering and Molecular Breeding of Hubei Province,Wuhan 430064,China)
关键词：
porcine oocytes;maturation;parthenogenetic embryos
摘  要：
The effect of 0.1% PVA,10% FBS and different concentration of EGF in the maturation media on maturation of porcine oocytes was studied.The results showed that : Addition of 0.1% PVA and 10% FBS in maturation medium had no significant effect on maturation rate,cleavage rate of 36th h and 6th day morula rate(P>0.05);Addition of different concen-tration of EGF in maturation medium had no significant effect on maturation rate(P>0.05),but addition of 30 and 40 ng·mL-1 EGF had higher cleavage rate of 36th h compared with that of 20 and 50 ng·mL-1(P<0.01),addition of 30,40 and 50 ng·mL-1 EGF in maturation medium had no significant effect on 6th day morula rate(P>0.05),and it was significantly higher than 20 ng·mL-1(P<0.01).In conclusion,addition of 0.1% PVA could instead of 10% FBS in maturation medium,addition of 30 ng·mL-1 EGF was the best for maturation of porcine oocytes and development of parthenogenetic embryoes.

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