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当前位置: 首页 > 文章 > 套皿法在猪卵母细胞体外成熟培养中的应用 湖北农业科学 2004,2004 (6) 71-73
Position: Home > Articles > Application of nest dish culture method on in vitro maturation of porcine oocytes Hubei Agricultural Sciences 2004,2004 (6) 71-73

套皿法在猪卵母细胞体外成熟培养中的应用

作  者：
李俊;田永祥;曹斌云;谷习文;郑新民
单  位：
西北农林科技大学动物科技学院;湖北省农业科学院生物技术研究所
关键词：
猪卵母细胞;套皿法;体外成熟;发育能力
摘  要：
试验表明,套皿无矿物油组卵母细胞成熟率与套皿矿物油组、单皿矿物油组差异不显著,单皿无矿物油组没有卵母细胞成熟;将各组成熟的卵母细胞分别进行体外受精,结果套皿无矿物油组卵母细胞的穿卵率、单精入卵率与套皿矿物油组、单皿矿物油组差异不显著,但雄原核形成率和卵裂率显著高于套皿矿物油组和单皿矿物油组(P<0.05)。套皿法有利于卵母细胞胞质成熟,促进胚胎生长发育,能够替代传统的单皿矿物油覆盖培养法。
译  名：
Application of nest dish culture method on in vitro maturation of porcine oocytes
作  者：
LI Jun1,TIAN Yong-xiang2,CAO Bin-yun1,GU Xi-wen2,ZHENG Xin-min2(1.Northwest Science and Technology University of Agriculture and Forestry,Shanx i,Yangling 712100,China: 2.Bio-technology Institute of Hubei Academy of Agricultural Science,Wuhan 430064 ,China)
关键词：
porcine oocytes;nest dish;IVM;development competence
摘  要：
To investigate the feasibility of nest dish without mineral oil culture method(nest dish method)on in vitro maturation(IVM)of porcine oocytes, we used nest dish or single dish. According to the using or no using of mineral oil, we divises the oocytes into four groups to culture:①nest dish without mineral oil, ②nest dish with mineral oil,③single dish without mineral oil,④single dish wit h mineral oil.The results showed no significant difference of maturation rate am ong group①?②and④.However, no matured oocytes were seen in group③.In order to study the effects of nest dish method on the subsequent development competence, we use the matured oocytes to fertilized in vitro.No significant effects can be seen on the percentage of sperm penetration, monospermy amog group①?②and ④.B ut the male pronuclear formation and cleavage rate of group①was significant hig her than group②and ④(P<0.05).Our study indicates that nest dish method can sub stitute for classical culture method covered with mineral oil, and it can also i mprove cytoplasm maturation and enhance embryo development competence.

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