中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 安徽省五华鸡J亚群禽白血病病毒gp85基因分子序列特征分析 中国畜牧兽医 2013,40 (11) 195-200
Position: Home > Articles > Sequence Feature Analysis of gp85 Gene of Avian Leukosis Virus Subgroup J in Wuhua Chickens in Anhui Province China Animal Husbandry & Veterinary Medicine 2013,40 (11) 195-200

安徽省五华鸡J亚群禽白血病病毒gp85基因分子序列特征分析

作  者：
戴银;赵瑞宏;胡晓苗;张丹俊;夏松林;盛昌树;杨文超;钟国发
单  位：
安徽省农科院畜牧兽医研究所;繁昌县五华鸡保种场;繁昌县动物疫病预防与控制中心;芜湖市动物疫病预防与控制中心
关键词：
J亚群禽白血病病毒;gp85基因;序列特征
摘  要：
为进一步了解J亚群禽白血病病毒(avian leukosis virus subgroup J,ALV-J)gp85基因的结构、功能及其遗传变异趋势,采用PCR方法克隆了安徽省地方品种五华鸡ALV-J gp85基因序列ALV-J-WH,并将该序列与GenBank数据库中登录的15个gp85基因序列进行了比对分析。结果显示,ALV-J-WH与所比对的氨基酸序列同源性介于85.9%~96.2%之间,与其同源性最高的是来自于国内ALV-J毒株的JN378888基因序列,进化树也证实两者亲缘关系较近;此外,相对其他ALV-J gp85氨基酸序列,ALV-J-WH在223位氨基酸后有10个氨基酸的缺失;对所有全长序列分析结果表明,共有71个可变氨基酸位点,10个高度变异位点,尤其是hr1和hr2区域分布较多。结果表明,ALV-J-WH与国内部分ALV-J毒株分离gp85基因来自共同的原始病毒,但其具有独特的序列特征;ALV-J gp85基因高度易变,部分高频率突变的氨基酸位点可能是ALV-J在抗体免疫选择压下的作用位点。
译  名：
Sequence Feature Analysis of gp85 Gene of Avian Leukosis Virus Subgroup J in Wuhua Chickens in Anhui Province
作  者：
DAI Yin;ZHAO Rui-hong;HU Xiao-miao;ZHANG Dan-jun;XIA Song-lin;SHENG Chang-shu;YANG Wen-chao;ZHONG Guo-fa;Institute of Animal Husbandry and Veterinary Science,Anhui Academy of Agricultural Sciences;Wuhu Center for Animal Disease Control and Prevention;Center for Animal Disease Control and Prevention of Fanchang County;Wuhua Chicken Breed Protection Company of Fanchang County;
关键词：
avian leukosis virus subgroup J;;gp85gene;;sequence feature
摘  要：
In order to further understand the structure,function and genetic variation of ALV-J gp85 gene,the gene was cloned by PCR in Anhui province,which named as ALV-J-WH.We compared ALV-J-WH with fifteen amino acid sequence of ALV-J gp85 genes in GenBank database.The results showed that the homology between ALV-J-WH and the other sequences ranged from 85.9% to 96.2%,the highest homology with ALV-J-WH were JN378888 sequences isolated from China.Evolution analysis further revealed that they had a close genetic relationship.Moreover,the deletion of a 10 amino acid was observed in the ALV-J-WH between position 223 and 224of amino acid sequence.Alignment of the full-length sequences revealed 71 variable amino acid sites,10 sites had high mutation frequency,expecially sites in hr1 and hr2 domains.The results confirmed that ALV-J-WH and partial genes of strains isolated from China evolved from common strains,but the sequence was unique. ALV-J gp85 gene was highly variable,and some amino acids with high degree of mutation frequency might be action sites of ALV-J antibody under immune selective pressure.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46