中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > Cu~(2+)Cd~(2+)对过氧化氢酶活性的影响及其荧光光谱研究 农业环境科学学报 2004,23 (06) 1086-1088
Position: Home > Articles > Effect of Cu~(2+) and Cd~(2+) on Activity and Fluorescence Spectrum of Catalase Journal of Agro-Environment Science 2004,23 (06) 1086-1088

Cu~(2+)Cd~(2+)对过氧化氢酶活性的影响及其荧光光谱研究

作  者：
赵艳红;袁小英;郭栋生
单  位：
山西大学化学化工学院;山西大学环境与资源学院
关键词：
Cu~(2+);Cd~(2+);过氧化氢酶;碘量法;荧光光谱法
摘  要：
分别采用碘量法和荧光光谱法研究了水溶液体系中重金属Cu2+、Cd2+对过氧化氢酶活性的影响。碘量法结果表明,Cu2+、Cd2+对过氧化氢酶既有激活作用也有抑制作用,这种作用与离子的浓度有关,而且在相同的浓度下,Cu2+对过氧化氢酶的作用较Cd2+强。荧光光谱法研究结果表明,Cu2+对过氧化氢酶的荧光有较强的猝灭作用,而Cd2+作用较弱,反映了2种离子对过氧化氢酶活性影响的机理可能不同。从荧光猝灭结果求出Cu2+和过氧化氢酶的结合常数K及结合位点数n,分别为K=18.42L·mol-1,n=0.5。
译  名：
Effect of Cu~(2+) and Cd~(2+) on Activity and Fluorescence Spectrum of Catalase
作  者：
ZHAO Yan hong1, YUAN Xiao ying2, GUO Dong sheng1 (1.School of Environment and Resources, Shanxi University, Taiyuan 030006,China; 2. School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China)
关键词：
Cu2+ ; Cd2+ ; catalase; iodometric method; fluorescence spectrum
摘  要：
The influence of Cu2+ and Cd2+ on the activity of catalase was studied by using the method of iodimetry. The relative activity and inhibition rate of Cu2+ and Cd2+ on catalase were calculated. Both Cu2+ and Cd2+ had the action of activiation and inhibition on catalase, which depended on the concentration of Cu2+ and Cd2+ . Moreover, Cu2+ had a stronger effect on catalase than Cd2+ in the same concentration. The effective mechanism was researched using fluorescence spectrum by scanning fluorescence spectrum and determing absorbance. The mechanisms of Cu2+ and Cd2+ affected catalase activity were different. Cu2+ might change the space structure of catalase, but Cd2+ had effect on the catalytic and binding radical. Based on the fluorescence quenching results, the binding constant (K) was 18.42 L· mol- 1 and the number of binding site (n) of catalase with Cu2+ was 0.5.

相似文章

导出引用
计量
文章访问数: 16
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46