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当前位置: 首页 > 文章 > 姜黄素抑制Cu~(2+)诱导的转APP_(695)基因SH-SY5Y细胞氧化损伤和凋亡作用 食品科学 2017,38 (3) 164-169
Position: Home > Articles > Inhibitory Effects of Curcumin on Cu~(2+)-Induced Oxidative Damage and Cell Apoptosis in Transgentic APP_(695) SH-SY5Y Cells FOOD SCIENCE 2017,38 (3) 164-169

姜黄素抑制Cu~(2+)诱导的转APP_(695)基因SH-SY5Y细胞氧化损伤和凋亡作用

作  者：
郑博闻;姜招峰;黄汉昌
单  位：
北京联合大学生物活性物质与功能食品北京市重点实验室
关键词：
阿尔茨海默病;Cu~(2+);氧化损伤;细胞凋亡;姜黄素;Nrf2/ARE信号通路
摘  要：
目的:研究Cu~(2+)诱导的转β-淀粉样前体蛋白(amyloid-β precursor protein,APP)基因SH-SY5Y(SHSY5Y-APP_(695))细胞氧化损伤、凋亡及姜黄素的抑制作用。方法:在姜黄素预保护和无姜黄素预保护条件下,50 μmol/L Cu~(2+)处理细胞24 h,测定细胞存活率、胞外乳酸脱氢酶(lactate dehydrogenase,LDH)水平、胞内活性氧(reactive oxygen,ROS)水平、线粒体膜电位、半胱氨酸天冬氨酸蛋白酶(caspase)-3、caspase-8和caspase-9活力,蛋白免疫印迹法测定核转录NF-E2相关因子2(NF-E2-related factor 2,Nrf2)Ser40位点磷酸化(p Ser40-Nrf2)水平以及血红素加氧酶(heme oxygenase,HO)-1蛋白表达水平。结果:与空白对照相比,Cu~(2+)损伤组细胞存活率降低,胞内ROS水平和胞外LDH活性升高,线粒体膜电位下降,caspase-9和caspase-3活性明显升高,p Ser40-Nrf2和HO-1含量增加,而姜黄素保护组细胞存活率升高,胞内ROS水平和LDH释放水平降低,线粒体膜电位恢复升高,caspase-9和caspase-3活性明显降低,p Ser40-Nrf2和HO-1表达量减少。结论:姜黄素在一定程度上减弱了Cu~(2+)诱导的氧化损伤和细胞凋亡作用。
译  名：
Inhibitory Effects of Curcumin on Cu~(2+)-Induced Oxidative Damage and Cell Apoptosis in Transgentic APP_(695) SH-SY5Y Cells
作  者：
ZHENG Bowen;JIANG Zhaofeng;HUANG Hanchang;Beijing Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University;
关键词：
Alzheimer's disease;;Cu~(2+);;oxidative damage;;cell apoptosis;;curcumin;;Nrf2-antioxidant response element pathway
摘  要：
Objective:This study was focused on the protective effect of curcumin on cellular oxidative damage and cell apoptosis induced by Cu~(2+) in human neuroblastoma SH-SY5 Y cells transfected by human amyloid-β precursor protein(APP)(SH-SY5Y-APP_(695)).Methods:SH-SY5Y-APP_(695) cells were treated with 50 μmol/L Cu~(2+) at 37℃ for 24 h with or without curcumin pre-protection.Cell viability was detected by cell counting kit-8(CCK-8).Extracellular lactate dehydrogenase(LDH),intracellular reactive oxygen species(ROS) and mitochondrial membrane potential were determined by commercial assay kits.The enzymatic activities of caspase-3,caspase-8 and caspase-9 were assessed.Phosphorylation levels of NF-E2-related factor 2(Nrf2) at Ser-40(pSer40-Nrf2) and HO-1 were detected by Western blot analysis.Results:Compared with the control group,Cu~(2+) administration led to decreased cell viability and mitochondrial membrane potential,increased levels of LDH,intracellular ROS and caspase-3,caspase-8 and caspase-9 activities,and elevated levels of pSer40-Nrf2 and HO-1.Conversely,curcumin increased cell viability and mitochondrial membrane potential,decreased the levels of LDH and intracellular ROS,significantly mitigated caspase-9 and caspase-3 activities,and reduced the expression levels pSer40-Nrf2 and HO-1.Conclusion:Curcumin can attenuate Cu~(2+)-induced cellular oxidative damage and cell apoptosis.

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