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当前位置: 首页 > 文章 > 哺乳动物(小鼠和绵羊)早期胚胎体外培养的研究——Ⅱ.不同培养液对小鼠和绵羊胚胎体外发育的影响 畜牧兽医学报 1984,15 (2) 6-11
Position: Home > Articles > STUDIES ON THE IN VITRO CULTURE OF EARLY MAMMALIAN EMBRYOS——Ⅱ.The Effect of Media on the Development of Mouse and Sheep Embryos In Vitro Chinese Journal of Animal and Veterinary Sciences 1984,15 (2) 6-11

哺乳动物(小鼠和绵羊)早期胚胎体外培养的研究——Ⅱ.不同培养液对小鼠和绵羊胚胎体外发育的影响

作  者：
刘云海;蒋世娥;朱成宽;朱裕鼎;郑丕留
摘  要：
869个2～4细胞小鼠胚胎分别在 TCM-199、SOF 和 PBS 液(Whittingh-am,1971)和经本试验改良的 TCM-199、SOF 及 PBS 液中进行培养。96小时以后,6种液中的胚胎发育以改良的 TCM-199最佳,其它依次为改良的 SOF和 PBS 以及未经改良的 PBS、SOF 和 TCM-199液。53个8～16细胞绵羊胚胎在改良的 TCM-199液和 SOF 液以及本试验未改良的原 PBS 液(Whittingham,1971)中进行培养。结果在改良的 TCM-199液和SOF 液之间未见显著差异,但这两种液与 PBS 液相比则差异均高度显著。
译  名：
STUDIES ON THE IN VITRO CULTURE OF EARLY MAMMALIAN EMBRYOS——Ⅱ.The Effect of Media on the Development of Mouse and Sheep Embryos In Vitro
作  者：
Liu Yunhai Jiang Shie Zhu Chengkuan Zhu Yuding Cheng Peilieu (Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing.China)
摘  要：
Eight hundred sixty nine 2 to 4-cell mouse embryos were cultured in TCM-199,SOF,PBS(Wh- ittingham,1971) and their modified media,for 96 hours.The modified TCM-199 was the best in the 6 media for embryo development,then followed by modified SOF,modified FBS,PBS,SOF and TCM-199 in order. Fifty three 8 to 16-cell sheep embryos were cultured in modified TCM-199,SOF and Whirring- hamts PBS for 7 days.No significant difference was found between TCM-199 and SOF,hut there were significant differences between the above 2 media and PBS. Improvement on the development of mouse embryos cultured in vitro has been resulted from the ad- dition of Na-lactate into PBS and both Na-laetate and Na-pyruvate into TCM-199.

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