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当前位置: 首页 > 文章 > HPLC法测定5种果汁中抗坏血酸的含量 安徽农业科学 2009,37 (4) 1412-1413
Position: Home > Articles > Determination of Ascorbic Acid Content in Five Fruit Juices by HPLC Journal of Anhui Agricultural Sciences 2009,37 (4) 1412-1413

HPLC法测定5种果汁中抗坏血酸的含量

作  者：
刘文海;范洁;刘丽莉
单  位：
湖南科技大学生命科学学院
关键词：
高效液相色谱;果汁;抗坏血酸
摘  要：
[目的]采用高效液相色谱法(HPLC)对5种水果中抗坏血酸含量进行测定和分析,进一步优化色谱条件。[方法]以脐橙(广西)、番茄(湖南)、沙田柚(广西)、苹果(河北)和猕猴桃(湖南)为试材,榨汁和离心后采用HPLC法测定了5种水果中抗坏血酸含量。色谱条件为ODS-C18色谱柱,磷酸钾溶液(pH值为6.0)为流动相,流速为1.0 ml/min,检测波长为260 nm,柱温为8℃。[结果]各水果果汁中抗坏血酸含量大小分别为:脐橙>猕猴桃>沙田柚>番茄>苹果。抗坏血酸在浓度0.005~0.750 g/L内有良好的线性关系,线性回归方程为y=158 558.187+18 923 522.816x(r=0.999 6),线性范围为0.05~7.50μg,标准偏差(s)为3 906.931,相对标准偏差(RSD)为0.040 96%。[结论]用所建立的HPLC法测定的结果准确度高、重现性好,适用于新鲜的果类等食品中抗坏血酸的快速定量分析。
译  名：
Determination of Ascorbic Acid Content in Five Fruit Juices by HPLC
作  者：
LIU Wen-hai et al(School of Life Science,Hunan University of Science and Technology,Xiangtan,Hunan 411201)
关键词：
HPLC;Fruit juices;Ascorbic acid
摘  要：
[Objective] The research aimed to determine and analyze the ascorbic acid content in 5 fruits by using HPLC,and further optimize the chromatographic conditions.[Method] With navel orange(Guangxi),tomato(Hunan),Shatian pomelo(Guangxi),apple(Hebei) and kiwifruit(Hunan) as tested materials,the ascorbic acid content in 5 fruits was determined by HPLC after juicing and centrifugation.The chromatographic conditions were: ODS-C18 column,potassium phosphate solution(pH=6.0) was used as mobile phase,flow velocity was 1.0 ml/min,and the detection wave length was 260 nm with the column temperature of 8 ℃.[Result] The content magnitude of ascorbic acid in each fruit juice in sequence was as below: navel orange > kiwifruit > Shatian pomelo > tomato > apple.The ascorbic acid had a good linear relation between the concentrations from 0.005 to 0.750 g/L and the linear regression equation was y= 158 558.187+18 923 522.816x(r=0.999 6),with the linear scope of 0.05-7.5 μg,standard deviation s=3 906.931 and the relative standard deviation(RSD) =0.040 96%.[Conclusion] The determined results by HPLC that was established in the test were in high accuracy and good reproducibility,and the method was suitable for rapid quantitative analysis of ascorbic acid in the fresh fruit and other foods.

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