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当前位置: 首页 > 文章 > 缺失ATP合酶和插入VHb基因对钝齿棒杆菌谷氨酸产量的影响 食品科学 2015,36 (7) 131-136
Position: Home > Articles > Effects of ATP Synthase Gene Deletion and VHb Gene Insertion on Production of Glutamic Acid in Corynebacterium crenatum FOOD SCIENCE 2015,36 (7) 131-136

缺失ATP合酶和插入VHb基因对钝齿棒杆菌谷氨酸产量的影响

作  者：
郑方亮;于亮;牟晓丛;刘鹏;刘宏生
单  位：
辽宁大学生命科学院
关键词：
钝齿棒杆菌;谷氨酸;ATP合酶;透明颤菌血红蛋白
摘  要：
为了提高钝齿棒杆菌生产谷氨酸的产量,以谷氨酸生产菌钝齿棒杆菌(Corynebacterium crenatum)T6-13为出发菌株,利用同源重组技术使其缺失ATP合酶基因(atp)并进一步插入透明颤菌血红蛋白基因(VHb),得到两株重组菌株T6-13-Δatp和T6-13-Δatp-tac-VHb,通过检测重组菌株谷氨酸产量及生长情况可以看出,在发酵32 h后,atp基因缺失菌株较出发菌株谷氨酸产出累积量高27.76%,但atp基因缺失导致菌体生长缓慢且无法到达出发菌株稳定期浓度,而插入VHb基因的atp基因缺失菌株的谷氨酸产出累积量较出发菌株高36.91%,且菌体的生长速率及稳定期浓度与出发菌株基本一致。
译  名：
Effects of ATP Synthase Gene Deletion and VHb Gene Insertion on Production of Glutamic Acid in Corynebacterium crenatum
作  者：
ZHENG Fangliang;YU Liang;MU Xiaocong;LIU Peng;LIU Hongsheng;Academy of Science, Liaoning University;
关键词：
Corynebacterium crenatum;;glutamic acid;;ATP synthase;;Vitreoscilla hemoglobin
摘  要：
In order to improve the yield of glutamic acid, we deleted the ATP synthase gene and inserted Vitreoscilla hemoglobin(VHb) gene into Corynebacterium crenatum genome by homologous recombination to acquire two recombinant Corynebacterium crenatum, T6-13-△atp and T6-13-△atp-tac-VHb. The yield of glutamic acid and growth of recombinant Corynebacterium crenatum were determined. The yield of glutamic acid produced by T6-13-△atp strain after 32 h of fermentation was 27.76% higher than that obtained from the original strain, although the recombinant strain grew more slowly and could not reach the concentration of the original strain at the stationary stage. Meanwhile, the yield of glutamic acid produced by T6-13-△atp-tac-VHb strain was 36.91% higher than that of the original strain during 32 h fermentation, and its growth rate and the glutamic acid concentration at the stable stage were the same as those observed for the original strain. These results may lay the foundation for constructing high-yield and stable industrial glutamic acid-producing strains.

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