当前位置: 首页 > 文章 > 钝齿棒杆菌精氨酸生物合成相关基因簇argCJBDFR的扩增及序列分析 中国生态农业学报 2006,14 (2) 32-36
Position: Home > Articles > Cloning and sequencing of the tandem arginine biosynthetic genes argCJBDFR from Corynebacterium crenatum A.S1.542. Chinese Journal of Eco-Agriculture 2006,14 (2) 32-36

钝齿棒杆菌精氨酸生物合成相关基因簇argCJBDFR的扩增及序列分析

作  者:
陈雪岚;许正宏;陶文沂;王正祥
单  位:
江南大学教育部工业生物技术重点实验室;江南大学工业微生物研究中心
关键词:
钝齿棒杆菌;精氨酸;基因簇;序列分析
摘  要:
采用PCR方法扩增钝齿棒杆菌精氨酸生物合成基因簇argCJBDFR,其序列长为6080bp,其中含有argJ、argB、argD、argF和argR5个完整的开放阅读框。序列和结构分析表明这5个结构基因编码的氨基酸序列与谷氨酸棒杆菌的相应基因编码的氨基酸高度同源,同源性分别为92%、95%、96%、97.5%和100%。
译  名:
Cloning and sequencing of the tandem arginine biosynthetic genes argCJBDFR from Corynebacterium crenatum A.S1.542.
作  者:
CHEN Xue-Lan,XU Zheng-Hong,TAO Wen-Yi(Key Laboratory of Industry Biotechnology,Ministry of Education,Southern Yangtze University,Wuxi 214036,China),WANG Zheng-Xiang(The Research Center of Industry Microbiology,Southern Yangtze University,Wuxi 214036,China)
关键词:
Corynebacterium crenatum,Aginine,Tandem genes,Sequence analysis
摘  要:
The tandem arginine biosynthetic genes argCJBDFR from Corynebacterium crenatum were amplified by PCR.Sequence analysis indicates that there are five open reading frames,including argJ,argB,argD,argF and argR in the 6080bp stretch.The amino acid sequences coded by argJBDFR genes show 92%,95%,96%,97.5% and 100% identity to those of Corynebacterium glutacimum,respectively.

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