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Position: Home > Articles > Purification and Identification of Aspergillus ficuum Inulinases FOOD SCIENCE 2007,28 (6) 188-192

Aspergillus ficuum菊粉酶的分离纯化与鉴定

作  者:
王静;金征宇;江波;徐学明
单  位:
江南大学食品学院
关键词:
菊粉酶;分离纯化;鉴定;Aspergillus ficuum
摘  要:
Aspergillus ficuum菊粉酶系经硫酸铵分级沉淀、透析脱盐、DEAE-Cellulose离子交换色谱、Sepharose CL-6B凝胶过滤色谱和制备电泳技术分离纯化,获得五个电泳纯酶:三个外切菊粉酶组分Exo-I、Exo-II、Exo-III和两个内切菊粉酶组分Endo-I、Endo-II。SDS-PAGE分析测得五种酶的分子量分别为:Exo-I:69961Da,Exo-II:39973Da,Exo-III:45561Da,Endo-I:33574Da,Endo-II:30769Da。
译  名:
Purification and Identification of Aspergillus ficuum Inulinases
作  者:
WANG Jing,JIN Zheng-yu,JIANG Bo,XU Xue-ming (School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China)
关键词:
inulinase;purification;identification;Aspergillus ficuum
摘  要:
Aspergillus ficuum inulinases were purified in a sequence of operations including ammonium sulfate fractionation, ion exchange chromatography on DEAE-Cellulose, column chromatography with Sepharose CL-6B and preparative electrophoresis. Five purified enzymes were thus obtained, two having an endo-type activity and the other being exo-actings. The five enzymes were identified as Exo-I, Exo-II, Exo-III, Endo-I and Endo-II, with MW(molecular weight) of 69961Da, 39973Da, 45561Da, 33574Da, 30769Da, respectively, determined by SDS-PAGE.

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