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马克斯克鲁维酵母菊粉酶的分离纯化和酶学性质研究

作  者:
纠敏;汪伦记
单  位:
河南科技大学食品与生物工程学院
关键词:
马克斯克鲁维酵母;菊粉酶;纯化;酶学性质
摘  要:
马克斯克鲁维酵母产生的孢外菊粉酶经超滤浓缩、DEAE-Cellulose阴离子交换色谱、SephadexG-100凝胶色谱分离纯化,得到两个菊粉酶组分ExoⅠ和ExoⅡ,I/S值分别为0.0249和0.0253,均属外切菊粉酶。ExoⅠ经聚丙烯酰胺凝胶鉴定为均一组分,分子量为85kD。ExoⅠ最适pH值为4.0,最适温度为60℃,Mn2+和Mg2+对酶活力有促进作用,而Cu2+、Fe2+对酶活力有抑制作用,ExoⅠ水解菊粉溶液的产物为果糖和少量葡萄糖。
译  名:
Purification and Enzymatic Properties of Inulinase from Kluyveromyces marxianus
作  者:
JIU Min,WANG Lun-ji(Food and Bioengineering Department,Henan University of Science and Technology,Luoyang 471003,China)
关键词:
Kluyveromyces marxianus;inulinase;purification;enzymatic property
摘  要:
Crude extracellular inulinase from Kluyveromyces marxianus was purified through sequential steps of ultrafiltration,DEAE-cellulose anion exchange chromatography and SephadexG-100 gel filtration to obtain two components,designated as Exo I and Exo II inulinase.The molecular weight of Exo I inulinase was 85 kD.The optimal reaction pH and temperature of Exo I were 4.0 and 60 ℃,respectively.In addition,Mn2+and Mg2+ could improve the activity of Exo I inulinase;in contrast,Cu2+ and Fe2+ exhibited a strong inhibition effect on Exo I inulinase activity.Enzymatic hydrolysates of inulin by Exo I inulinase were fructose and a small amount of glucose.

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