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当前位置: 首页 > 文章 > 基于糙皮侧耳转录组测序挖掘降解木本基质的候选功能基因 吉林农业大学学报 2018 (2) 164-170
Position: Home > Articles > Detection of Candidate Genes for Degradation of Woody Substrates by Pleurotus ostreatus (Jacq.: Fr.) Kummer Based on Transcriptome Sequencing Journal of Jilin Agricultural University 2018 (2) 164-170

基于糙皮侧耳转录组测序挖掘降解木本基质的候选功能基因

作  者：
金银萍;逄世峰;焉石;王博;郭靖;王英平
关键词：
糙皮侧耳;转录组;高通量测序;差异基因
摘  要：
采用Illumina Hi Seq 4000 SBS高通量测序平台对糙皮侧耳[Pleurotus ostreatus(Jacq.:Fr.)Kummer]的转录组进行测序,挖掘糙皮侧耳降解木质基质的候选功能基因。共得到糙皮侧耳转录本序列64 228条,单基因序列46 417条。使用Blast工具将转录本序列比对到NR、Swissprot、KEGG、String、Pfam等数据库中,并有33 736条序列得到注释。通过对差异基因的对比和筛选,得到1 408条差异表达基因,其中有1 183条基因为上调基因,通过GO富集分析表明,差异基因显著富集于木质素分解代谢过程、锰过氧化物酶活性、苯丙素分解代谢过程中。将差异基因比对到KEGG数据库中,共有313条得到注释的序列,对其进行Pathway富集分析,证明差异基因显著富集于氨基苯甲酸降解、色氨酸代谢、β-丙氨酸代谢等通路中。c27028_g1、c28624_g1、c30793_g1、c30849_g1等基因与木质素降解过程相关性较高。c24893_g1、c29559_g1、c29956_g2等基因与纤维素降解过程相关性较高。
译  名：
Detection of Candidate Genes for Degradation of Woody Substrates by Pleurotus ostreatus (Jacq.: Fr.) Kummer Based on Transcriptome Sequencing
作  者：
WEI Shanshan;HE Junyang;SONG Qian;WANG Jianguo;GENG Nannan;DU Yarong;SONG Ruiqing;School of Forestry,Northeast Forestry University;
关键词：
Pleurotu sostreatus;;transcriptome;;high throughput sequencing;;differential gene
摘  要：
the translocation group transcriptome of Pleurotus ostreatus( Jacq.: Fr.) Kummer was sequenced by Illumina Hi Seq 4000 SBS Kit,and the functional genes of wood plexus were degraded.A total of 64 228 sequences of transcript sequences were obtained. A total of 46 417 single gene sequences were obtained. Blast software was used to compare transcript sequences with databases such as NR,Swissprot,KEGG,String and Pfam,and 33 736 sequences were annotated. According to the FPKM value of the gene,1 408 different genes were selected and 1 183 genes were up-regulated.Through the GO enrichment analysis,the differential gene was significantly enriched in lignin catabolism process,manganese peroxidase activity and phenylpropanine catabolism process. The differential gene was compared with the KEGG database,and a total of 313 sequences were obtained. The differential gene was significantly enriched in the degradation process of aminobenzoate degradation,tryptophan metabolism,beta-alanine metabolism,arginine and proline metabolism,pentose and glucuronate interconversions and other channels. Among them,c27028 _ g1,c28624 _ g1,c30793 _ g1 and c30849_g1 are highly correlated with lignin degradation process. C24993_g1,c29559_g1 and c29956_g2 are highly correlated with cellulose degradation process.

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