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Position: Home > Articles > High Performance Liquid Chromatography Determination of Endogenous Hormones in Mature Leaves of Agavybrid No.11648 Journal of Anhui Agricultural Sciences 2011,39 (22) 13266-13267+13289

剑麻叶片内源激素高效液相色谱测定方法研究

作  者:
俞奔驰;黄富宇;吕平;梁立娟;彭静茹;张小玲
单  位:
广西农垦山圩农场;广西壮族自治区亚热带作物研究所;广西壮族自治区木薯研究所
关键词:
高效液相色谱;剑麻;成熟叶片;内源激素;测定方法
摘  要:
[目的]探讨剑麻叶片内源激素的高效液相色谱(HPLC)测定方法。[方法]融合酶联免疫法(ELISA)的激素提取方法,对剑麻成熟叶片内源激素——赤霉素(GA3)、3-吲哚乙酸(IAA)、脱落酸(ABA)和玉米素(ZT)进行了HPLC测定。[结果]该流程可联合测定GA3I、AA、ABA和ZT,回收率分别为78.81%、104.96%8、1.24%和100.55%,且保留时间稳定,试验重现性好。[结论]整个提取、分离、分析流程较快速、准确,适合剑麻成熟叶片内源激素的分析测定。
译  名:
High Performance Liquid Chromatography Determination of Endogenous Hormones in Mature Leaves of Agavybrid No.11648
作  者:
YU Ben-chi et al(Guangxi Institute of Subtropical Crops,Nanning,Guangxi 530001)
关键词:
High performance liquid chromatography;Agavybrid No.11648;Mature leaves;Endogenous hormones;Determination method
摘  要:
[Objective] The aim was to study the high performance liquid chromatography(HPLC) determination of endogenous hormones in agavybrid No.11648 leaves.[Method] Combining the hormone extraction methods of enzyme linked immunoassay,Gibberellin acid(GA3),3-indoleacetic acid(IAA),abscisic acid(ABA) and zeatin(ZT) in mature leaves of agavybrid No.11648 were determined by HPLC.[Result] The procedure could analyze the GA3,IAA,ABA and ZT in combination,and their recoveries were 78.81%,104.96%,81.24% and 100.55% respectively with stable retention time and good repeatability.[Conclusion] The procedure is simple,rapid and accurate,so it is suitable for the efficient determination of endogenous hormone in mature leaves of agavybrid No.11648.

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