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Position: Home > Articles > Screening of RAPD Markers for Sterility Gene of Nucleo-cytoplasmic Interaction in Mitochondrial DNA of Rice Acta Agronomica Sinica 2003,30 (6) 899-902

核质互作型水稻线粒体不育基因的RAPD标记筛选

作  者:
裴得胜;蔡平钟;李名扬;向跃武;阎文昭;张志雄;侯磊;张志勇;裴炎
单  位:
西南农业大学生物技术中心;四川省农业科学院生物技术核技术研究所
关键词:
水稻雄性不育;线粒体DNA;RAPD分析;RAPD标记
摘  要:
通过单因素的改变对核质互作型水稻线粒体DNA的RAPD反应条件进行优化 ,并在最佳条件下用OPERON公司OPA~OPM系列共 2 6 0个随机引物筛选水稻Ⅱ 32不育系、保持系、杂种一代线粒体DNA的差异片段 ,结果共获得 2 2个差异带。其中OPH19 180 0、OPJ0 9 4 0 0、OPJ18 14 0 0、OPJ18 10 0 0、OPJ2 0 30 0这几个特异片段可能与水稻雄性不育相关。我们正在用分子检测确定这些标记与细胞质雄性不育的关系。
译  名:
Screening of RAPD Markers for Sterility Gene of Nucleo-cytoplasmic Interaction in Mitochondrial DNA of Rice
作  者:
PEI De-Sheng 1,2 CAI Ping-Zhong 1 LI Ming-Yang 2 XIANG Yue-Wu 1 YAN Wen-Zhao 1 ZHANG Zhi-Xiong 1 HOU Lei 2 ZHANG Zhi-Yong 1 PEI Yan 2 ( 1 Institute of Biotechnological & Nuclear Technich, Sichuan Academy of Agricultural Sciences, Chengdou,Sichuan 610066, 2Center of Biotechnology, Southwest Agricultural University, Chongqing 400716,China)
关键词:
Rice male sterility; Mitochondrial DNA; RAPD analysis; RAPD marker
摘  要:
The reaction conditions of RAPD for mitochondrial DNA of nucleo-cytoplasmic interaction rice were optimized by changing a single factor. Under the optimum conditions, Ⅱ-32 male sterility line, maintainer line and F 1 hybrid mitochondrial DNA were screened by 260 RAPD primers from OPA to OPM. As a result, 22 differential fragments were found. Among them, OPH19-1800, OPJ09-400, OPJ18-1400, OPJ18-1000 and OPJ20-300 might have relation with rice male sterility. For further study, the relationship between the markers and cytoplasmic male sterility would be studied.

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