作 者：
何正礼;储静华;金洪效;毛洪先;叶爱红;陈志森;张瑞义;张大隆
摘 要：
显微镜下观察猪肺炎支原体纯培养物与56℃灭活30分钟的待检血清混合,经在37℃培养24—48小时,涂片,以瑞氏染色后镜检。试验的方法有二:〈1〉无菌小试管中装0.4毫升纯培养旺盛的猪肺炎支原体,加0.1毫升灭活待检血清,加橡皮塞,混合培养。〈2〉适宜猪肺炎支原体生长的液体培养基0.36毫升,种入生长旺盛的、纯粹的猪肺炎支原体新鲜培养物0.04毫升,加灭活待检血清0.1毫升,加橡皮塞,混合培养。第〈1〉法凝集较快些,但凝集粒内菌体清晰度不如第〈2〉法。第〈2〉法凝集慢些,但菌体多系新增殖的,故在凝集粒内较为清晰。人工感染54只猪,在经X射线透视发现病变阴影后8天,可出现血清凝集阳性。一个月后达到高峰,並维持六周左右,其后逐渐下降,直至五个月,纵然病变完全消失(X射线透视阴性),仍有可见的阳性凝集反应。经采集野外感染猪群的猪血清共116份,X射线透视阳性者100份,作微粒凝集反应出现阳性者82份,符合率为82％。X射线透视阴性者16份,微粒凝集反应阴性者12份,符合率为75％。所以有此出入,盖因血清中凝集素的出现较X射线透视出现病变阴影为慢,又当病变消失,X射线透视转为阴性时,尚有一段时间凝集反应仍为阳性之故。
译 名：
MICROAGGLUTINATION TEST FOR THE DIAGNOSIS OF SWINE MYCOPLASMAL PNEUMONIA AND THE IDENTIFICATION OF MYCOPLASMAS
作 者：
Ho Cheng-lee, Chu Tsinghua, Chin Hunghsiao, Mao Hungsien, Yeh Aihung, Chen Chihshen, Chang Shuii, Chang Dalung. (Research Group of Swine Mycoplasmal Pneumonia, Institute of Animal Husbandry and Veterinary Medicine, Kiangsu Academy of Agricultural Science, Nanking, People's Republic of China)
摘 要：
Summary In order to save time in preparing the poorly yielded antigen from the fastidious growth culture of Mycoplasma hyopneumoniae, we directly used the pure culture as an antigen instead. To overcome the slow reactiveness of the mycoplasma-infected pig's sera in the ordinary agglunation test, we incubated the mixture of the tested serum and the culture for 24-48 hours, and examined the stained smears of the mixture under microscope. Two procedures were adopted: (1)0.4 ml of fresh pure luxuriant culture of Mycoplasma hyopneumoniae was added to a small sterile vial containing 0.1 ml of tested serum inactivated by 56℃ for 30 minutes, mixed well, and incubated in 37℃. The vial was stoppered with rubber cap to prevent evaporation. After 24-48 hourst' incubation, smears were made and stained with Wright's stain for microscopic examination. (2) 0.36 ml of modified Switzer's medium (KM~2), 0.04 ml of fresh pure luxuriant culture and 0.1 ml of tested serum were mixed well in a sterile small vial, and incubated in 37℃ 24-48 hours. Made smears and examined as in (1). The positive reactions appeared earlier in (1) than in (2), while the mycoplasmal cells in the agglutinated clumps were clearer in (2)than in (1). All the serum samples from 54 experimentally infected pigs showed positive reactions in the microagglutination test. A great majority of the positive reactions appeared 8 days after the occurrences of the shadows of lung lesions detected by X-ray. A month later the intensities of reactions reached a peak, maintained for about 6 weeks, and declined thereafter. 5 months Iater positive reactions were still detectable, even when the lung lesions were no longer detectable by X-ray examination. Out of 100 serum samples collected from pigs with lung lesions detected by X-ray in field infected herds,82(82%) showed positive reactions in the microagglutimation test, and out of 16 serum samples collected from pigs without lung lesions by X-ray detection 12 (75%) were negative in the microagglutination test. Antisera against the Annin (a county name in Gansu province) strain of M.hyopneumoniae showed positive reactions with 20 strains of Mycoplasmas colleted from laboratories in differant provinces of China, but did not react with M. hyorhinis, M.synouiae and Acholeplasma laidlawii. The J strain of M. hyopneumoniae from U.S.A. also reacted positively with hyperimmune antiserum against the Annin strain of M. hyopneumoniae.
